The influence of dehydroepiandrosterone on effector functions of neutrophils

Autor(es):Mostrar menos -

Brauer, Veronica Soares ^[1] ; Zambuzi, Fabiana Albani ^[1] ; Espindola, Milena Sobral ^[1] ; Cavalcanti Neto, Marinaldo Pacifico ^[1] ; Borges Prado, Morgana Kelly ^[1] ; Cardoso, Priscilla Mariane ^[1] ; Soares, Luana Silva ^[1] ; Galvao-Lima, Leonardo Judson ^[1] ; Leopoldino, Andreia Machado ^[1] ; de Barros Cardoso, Cristina Ribeiro ^[1] ; Frantz, Fabiani Gai ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FCFRP, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Pharmaceutical Sciences; v. 57, 2021.
• Citações Web of Science: 0

Resumo

Dehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal glands, gonads and brain. It is a precursor to sex hormones and also is known to have immune modulatory activity. However, little is known about the relationship between DHEA and neutrophils and thus our study evaluates the influence of DHEA in the effector functions of neutrophils. Human neutrophils were treated in vitro with DHEA and further infected with Salmonella enterica serovar Typhimurium. The treatment of neutrophils with 0.01 mu M of DHEA increased the phagocytosis of Salmonella independent of TLR4 as the treatment did not modulate the TLR4 expression. Additionally, DHEA caused a decrease in ROS (reactive oxygen species) production and did not influence the formation of the neutrophil extracellular trap ( NET). Steroid treated neutrophils, infected or stimulated with LPS (lipopolysaccharide), showed reduced production of IL-8, compared to untreated cells. Also, the protein levels of p-NF.B were decreased in neutrophils treated with DHEA, and this reduction could explain the reduced levels of IL-8. These results led us to conclude that the steroid hormone DHEA has important modulatory functions in neutrophils. (AU)

• Processo FAPESP: 11/12199-0 - Estudo de disfunções na resposta imune inata em pacientes HIV+: correlação com alterações imunológicas e epigenéticas
• Beneficiário: Fabiani Gai Frantz
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores