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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The influence of dehydroepiandrosterone on effector functions of neutrophils

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Author(s):
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Brauer, Veronica Soares [1] ; Zambuzi, Fabiana Albani [1] ; Espindola, Milena Sobral [1] ; Cavalcanti Neto, Marinaldo Pacifico [1] ; Borges Prado, Morgana Kelly [1] ; Cardoso, Priscilla Mariane [1] ; Soares, Luana Silva [1] ; Galvao-Lima, Leonardo Judson [1] ; Leopoldino, Andreia Machado [1] ; de Barros Cardoso, Cristina Ribeiro [1] ; Frantz, Fabiani Gai [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, FCFRP, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Brazilian Journal of Pharmaceutical Sciences; v. 57, 2021.
Web of Science Citations: 0
Abstract

Dehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal glands, gonads and brain. It is a precursor to sex hormones and also is known to have immune modulatory activity. However, little is known about the relationship between DHEA and neutrophils and thus our study evaluates the influence of DHEA in the effector functions of neutrophils. Human neutrophils were treated in vitro with DHEA and further infected with Salmonella enterica serovar Typhimurium. The treatment of neutrophils with 0.01 mu M of DHEA increased the phagocytosis of Salmonella independent of TLR4 as the treatment did not modulate the TLR4 expression. Additionally, DHEA caused a decrease in ROS (reactive oxygen species) production and did not influence the formation of the neutrophil extracellular trap ( NET). Steroid treated neutrophils, infected or stimulated with LPS (lipopolysaccharide), showed reduced production of IL-8, compared to untreated cells. Also, the protein levels of p-NF.B were decreased in neutrophils treated with DHEA, and this reduction could explain the reduced levels of IL-8. These results led us to conclude that the steroid hormone DHEA has important modulatory functions in neutrophils. (AU)

FAPESP's process: 11/12199-0 - Elucidation of innate immune response dysfunction of HIV patients: the underlying mechanisms amongst immunological and epigenetic modifications
Grantee:Fabiani Gai Frantz
Support Opportunities: Research Grants - Young Investigators Grants