Quantification of torque teno virus (TTV) DNA in saliva and plasma samples in patients at short time before and after kidney transplantation

Autor(es):Mostrar menos -

Batista, Alexandre Mendes ^[1] ; Caetano, Matheus W. ^{[2, 1]} ; Stincarelli, Maria A. ^[3] ; Mamana, Ana C. ^[1] ; Zerbinati, Rodrigo Melim ^[1] ; Sarmento, Dmitry J. S. ^{[2, 4]} ; Gallottini, Marina ^[2] ; Caixeta, V, Rafael A. ; Medina-Pestana, Jose ^[5] ; Hasseus, Bengt ^[6] ; Zanella, Louise ^[7] ; Tozetto-Mendoza, Tania R. ^[1] ; Giannecchini, Simone ^[3] ; Braz-Silva, Paulo H. ^{[1, 8]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Inst Trop Med Sao Paulo, Lab Virol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil

^[3] Univ Florence, Dept Expt & Clin Med, Viale Morgagni 48, I-50134 Florence - Italy

^[4] State Univ Paraiba, Dept Oral Med, Araruna - Brazil

^[5] Univ Fed Sao Paulo, Sch Med, Kidney & Hypertens Hosp, Div Renal Transplantat, Sao Paulo - Brazil

^[6] Univ Gothenburg, Inst Odontol, Dept Oral Med & Pathol, Gothenburg - Sweden

^[7] Univ La Frontera, Lab Integrat Biol LIBi, Sci & Technol Bioresource Nucleus Ctr Excellence, Temuco - Chile

^[8] Caixeta, Rafael A., V, Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL MICROBIOLOGY; v. 14, n. 1 JAN 1 2022.
• Citações Web of Science: 0

Resumo

Background Several reports have proposed that the viral load of torque teno virus (TTV) in plasma is a biomarker of immune function in solid organ transplantation (SOT) and in allogeneic hematopoietic stem cell transplantation. Additionally, for the latter one, TTV-DNA quantification in saliva has also been suggested. Aim to investigate the correlation between the TTV viral load and immune function in paired saliva and plasma samples in patients on kidney transplantation. Materials and Methods TTV-DNA viral load was quantified in paired samples of saliva and plasma from 71 patients before and a short-time after renal-transplantation by real-time PCR. Results The data obtained from 213 paired samples showed a slight consistency in the comparison between saliva and plasma, with prevalence of TTV-DNA being 58%, 52% and 60% in saliva samples and 60%, 73% and 90% in plasma samples before and at 15-20 and 45-60 days after transplantation, respectively. Additionally, a high TTV viral load was observed in plasma at 15-20 and 45-60 days after transplantation compared to that observed in saliva at the same time. Conclusions Overall, monitoring TTV-DNA in saliva samples could be an additional fast non-invasive option to assess the immune functionality in SOT populations. (AU)

• Processo FAPESP: 21/03563-2 - Excreção oral e viremia de torque teno virus (TTV) em receptores de transplante renal
• Beneficiário: Matheus Willian Caetano
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/07778-8 - Impacto do transplante renal na saúde bucal e no manejo odontológico
• Beneficiário: Marina Helena Cury Gallottini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/07727-9 - Avaliação do ambiente imunoregulatório de lesões potencialmente malignas de boca, carcinomas de boca e orofaringe relacionados ou não ao HPV.
• Beneficiário: Paulo Henrique Braz da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular