| Texto completo | |
| Autor(es): |
Gomes, Brenda P. F. A.
[1]
;
Berber, Vanessa B.
[1]
;
Marinho, Ariane C. S.
[1]
;
Louzada, Lidiane M.
[1]
;
Arruda-Vasconcelos, Rodrigo
[1]
;
Passini, Maicon R. Z.
[1]
;
Lopes, Erica M.
[1]
;
Pecorari, Vanessa G. A.
[1, 2]
;
Chen, Tsute
[3]
;
Paster, Bruce J.
[4]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] State Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Restorat Dent, Div Endodont, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Paulista UNIP, BioStat, Sao Paulo - Brazil
[3] Forsyth Inst, Dept Mol Genet, Cambridge, MA - USA
[4] Forsyth Inst, Microbiol Dept, Cambridge, MA - USA
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF PERIODONTAL RESEARCH; DEC 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Background This study was conducted to compare the microbiomes, the levels of lipopolysaccharides (LPS), lipoteichoic acid (LTA), and cytokines (interleukin {[}IL]-1 beta and tumor necrosis factor-alpha {[}TNF-alpha]), before and after chemomechanical preparation (CMP) of the root canals (RC) and their associated periodontal pockets (PP) in teeth with combined EPL. Materials Samples were taken from 10 RC and PP, before and after CMP. The microbiomes (next-generation sequencing, V3-V4 hypervariable region of the 16S rRNA gene), microbiome diversity (bioinformatics analyses), LPS (limulus amebocyte lysate), LTA, IL-1 beta, and TNF-alpha (ELISA) were evaluated. A statistical analysis was performed with significance level set at 5%. Results The most abundant phyla in both sites were Firmicutes and Proteobacteria. Comparative studies of bacterial genera species revealed that some increased and others decreased after CMP at both sites. A 3% reduction in Gram-negative bacteria (RC) and a 4% increase in Gram-positive bacteria (PP) were detected. LPS levels were 4.4 times higher in PP than in the RC. LTA was detected in all samples investigated. Higher levels of IL-1 beta and TNF-alpha were detected in both sites at baseline. After CMP, LPS, LTA, IL-1 beta and TNF-alpha were reduced in both sites. Conclusion The microbial community in the RC and PP in teeth with combined EPL indicated a similarity between both sites. CMP effectively reduced the microbial load and the LPS levels from teeth with EPL, and consequently diminished the cytokine levels. The reduction in LTA levels in the RC and PP proved challenging. (AU) | |
| Processo FAPESP: | 17/25242-8 - Monitoramento clínico, microbiológico e imunológico de pacientes submetidos à terapia endodôntica com diagnóstico de Pulpite irreversível |
| Beneficiário: | Rodrigo Arruda Vasconcelos |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 14/01655-3 - Caracterização microbiana e imunológica de lesões endo-periodontais |
| Beneficiário: | Brenda Paula Figueiredo de Almeida Gomes |
| Modalidade de apoio: | Bolsas no Exterior - Pesquisa |
| Processo FAPESP: | 19/10755-5 - Investigação clínica de modulações epigenéticas em polpas dentais com diagnóstico de pulpite irreversível |
| Beneficiário: | Rodrigo Arruda Vasconcelos |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 15/23479-5 - Microbiomas e aspectos imunobiológicos nas infecções endodônticas |
| Beneficiário: | Brenda Paula Figueiredo de Almeida Gomes |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/19300-0 - Investigação dos aspectos clínicos, microbiológicos e imunológicos de dentes com diagnóstico de lesão periodontal primária com envolvimento endodôntico secundário |
| Beneficiário: | Lidiane Mendes Louzada |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |