Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response

Autor(es)

Biagi Jr, Carlos Alberto Oliveira ; Cury, Sarah Santiloni ^[1] ; Alves, Cleidson Padua ^[2] ; Rabhi, Nabil ^[3] ; Silva Jr, Wilson Araujo ; Farmer, Stephen R. ^[3] ; Carvalho, Robson Francisco ^[1] ; Batista Jr, Miguel Luiz

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, BR-18618689 Botucatu - Brazil

^[2] Univ Cologne, Med Fac, Dept Translat Gen, D-50923 Cologne - Germany

^[3] Boston Univ, Sch Med, Dept Biochem, Boston, MA 02215 - USA

• Tipo de documento: Artigo Científico
• Fonte: CELLS; v. 10, n. 11 NOV 2021.
• Citações Web of Science: 0

Resumo

Adipose tissue has been classified based on its morphology and function as white, brown, or beige/brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but also by their cellular origin, adaptation to metabolic stress, and plasticity. Here, we performed an in-depth analysis of publicly available single-nuclei RNAseq from adipose tissue and utilized a workflow template to characterize adipocyte plasticity, heterogeneity, and secretome profiles. The reanalyzed dataset led to the identification of different subtypes of adipocytes including three subpopulations of thermogenic adipocytes, and provided a characterization of distinct transcriptional profiles along the adipocyte trajectory under thermogenic challenges. This study provides a useful resource for further investigations regarding mechanisms related to adipocyte plasticity and trans-differentiation. (AU)

• Processo FAPESP: 18/20905-1 - TLR4 como crossroad da inflamação e metabolismo energético: Papel do tecido adiposo na síndrome de caquexia
• Beneficiário: Miguel Luiz Batista Junior
• Modalidade de apoio: Bolsas no Exterior - Pesquisa