Proteomic insight of seminal plasma in spinal cord injured men submitted to oral probenecid treatment for improved motility

Autor(es)

Camargo, Mariana ^[1] ; Ibrahim, Emad ^[2] ; Aballa, Teodoro C. ^[2] ; Cardozo, Karina H. M. ^[3] ; Carvalho, Valdemir M. ^[3] ; Lynne, Charles M. ^[2] ; Brackett, Nancy L. ^[2] ; Bertolla, Ricardo P. ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Div Urol, Dept Surg, Sao Paulo Hosp, R Embau 231, BR-04039060 Sao Paulo - Brazil

^[2] Univ Miami, Miller Sch Med, Miami Project Cure Paralysis, Miami, FL 33136 - USA

^[3] Fleury Grp, Sao Paulo - Brazil

^[4] Hosp Sao Paulo, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SPINAL CORD MEDICINE; v. 44, n. 6, p. 966-971, NOV 2 2021.
• Citações Web of Science: 0

Resumo

Objective: To observe the seminal plasma proteomic composition in men with spinal cord injury orally treated with probenecid, in order to observe pathways associated with increased sperm motility. Study design: Prospective study. Setting: Miami Project to Cure Paralysis - University of Miami/Miller School of Medicine. Participants: Nine men with spinal cord injury, who agreed to participate in the study. Intervention: Oral treatment with probenecid - 500 mg per day for one week, then 500 mg twice daily {[}1000 mg total] per day for three weeks. Outcome measures: Semen analysis as per WHO 2010 guidelines, and seminal plasma proteomics analysis by LC-MS/MS. Results: In total, 783 proteins were identified, of which, 17 were decreased, while 6 were increased after treatment. The results suggest a new pathway that could be treated by the decrease of biglycan after probenecid treatment. Conclusion: Oral treatment with probenecid is able to alter the seminal plasma proteome, in pathways that explain decreased innate immune response. (AU)

• Processo FAPESP: 12/15039-7 - Efeito da varicocele e de seu tratamento nas vias proteômicas e lipidômicas e no estresse oxidativo seminal em adultos.
• Beneficiário: Mariana Camargo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/17185-6 - Análise proteômica confirmatória de biomarcadores de alterações funcionais dos espermatozóides e estresse oxidativo seminal
• Beneficiário: Ricardo Pimenta Bertolla
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/14346-7 - Lesão medular e infertilidade masculina: efeito do tratamento com probenecida sobre a toxicidade seminal de origem prostática.
• Beneficiário: Mariana Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular