Proteomic insight of seminal plasma in spinal cord injured men submitted to oral probenecid treatment for improved motility

Author(s)

Camargo, Mariana ^[1] ; Ibrahim, Emad ^[2] ; Aballa, Teodoro C. ^[2] ; Cardozo, Karina H. M. ^[3] ; Carvalho, Valdemir M. ^[3] ; Lynne, Charles M. ^[2] ; Brackett, Nancy L. ^[2] ; Bertolla, Ricardo P. ^{[4, 1]}

Affiliation

^[1] Univ Fed Sao Paulo UNIFESP, Div Urol, Dept Surg, Sao Paulo Hosp, R Embau 231, BR-04039060 Sao Paulo - Brazil

^[2] Univ Miami, Miller Sch Med, Miami Project Cure Paralysis, Miami, FL 33136 - USA

^[3] Fleury Grp, Sao Paulo - Brazil

^[4] Hosp Sao Paulo, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF SPINAL CORD MEDICINE; v. 44, n. 6, p. 966-971, NOV 2 2021.
• Web of Science Citations: 0

Abstract

Objective: To observe the seminal plasma proteomic composition in men with spinal cord injury orally treated with probenecid, in order to observe pathways associated with increased sperm motility. Study design: Prospective study. Setting: Miami Project to Cure Paralysis - University of Miami/Miller School of Medicine. Participants: Nine men with spinal cord injury, who agreed to participate in the study. Intervention: Oral treatment with probenecid - 500 mg per day for one week, then 500 mg twice daily {[}1000 mg total] per day for three weeks. Outcome measures: Semen analysis as per WHO 2010 guidelines, and seminal plasma proteomics analysis by LC-MS/MS. Results: In total, 783 proteins were identified, of which, 17 were decreased, while 6 were increased after treatment. The results suggest a new pathway that could be treated by the decrease of biglycan after probenecid treatment. Conclusion: Oral treatment with probenecid is able to alter the seminal plasma proteome, in pathways that explain decreased innate immune response. (AU)

• FAPESP's process: 12/15039-7 - Varicocele effect and its treatment in proteomic and lipidomic pathways and in seminal oxidative stress in adults.
• Grantee: Mariana Camargo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/17185-6 - Confirmatory proteomic analysis of biomarkers of sperm functional alteration and seminal oxidative stress
• Grantee: Ricardo Pimenta Bertolla
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/14346-7 - Spinal cord injury and male infertility: effect of probenecid treatment on seminal toxicity of prostatic origin.
• Grantee: Mariana Camargo
• Support Opportunities: Regular Research Grants