Alginate-amphotericin B nanocomplexes covered by nanocrystals from bacterial cellulose: physico-chemical characterization and in vitro toxicity

Autor(es)

Soeiro, Victoria Soares ^{[1, 2]} ; Silva-Carvalho, Ricardo ^[1] ; Martins, Daniela ^[1] ; Parpot, Pier ^{[1, 3]} ; Grotto, Denise ^[4] ; Chaud, Marco Vinicius ^[5] ; Portela da Gama, Francisco Miguel ^[1] ; Jozala, Angela F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Minho, CEB Ctr Biol Engn, Campus Gualtar, P-4710057 Braga - Portugal

^[2] Univ Sorocaba, LAMINFE Lab Ind Microbiol & Fermentat Proc, Sorocaba - Brazil

^[3] Gualtar, Ctr Chem, Campus Gualtar, P-4710057 Braga - Portugal

^[4] Univ Sorocaba, Lapetox Lab Toxicol Res, Sorocaba - Brazil

^[5] Univ Sorocaba, LABNUS Biomat & Nanotechnol Lab, Sorocaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 DEC 14 2021.
• Citações Web of Science: 0

Resumo

Nanocomplexes systems made up natural poylymers have pharmacotechnical advantages such as increase of water solubility and a decrease of drugs toxicity. Amphotericin B (AmB) is a drug apply as anti-leishmanial and anti-fungal, however it has low water solubility and high toxicity, limiting its therapeutic application. With this in mind, the present study aimed to produce nanocomplexes composed by alginate (Alg), a natural polymer, with AmB covered by nanocrystals from bacterial cellulose (CNC). For this reason, the nanocomplexes were produced utilizing sodium alginate, amphotericin B in a borate buffer (pH 11.0). The CNC was obtained by enzymatic hydrolysis of the bacterial cellulose. To CNC cover the nanocomplexes 1 ml of the nanocomplexes was added into 1 ml of 0.01% CNC suspension. The results showed an ionic adsorption of the CNC into the Alg-AmB nanocomplexes surface. This phenomena was confirmed by an increase in the particle size and PDI decrease. Besides, nanocomplexes samples covered by CNC showed uniformity. The amorphous inclusion of AmB complex into the polysaccharide chain network in both formulations. AmB in the nanocomplexes was in supper-aggregated form and showed good biocompatibility, being significantly less cytotoxic in vitro against kidney cells and significantly less hemolytic compared to the free-drug. The in vitro toxicity results indicated the Alg-AmB nanocomplexes can be considered a non-toxic alternative to improve the AmB therapeutic effect. All process to obtain nanocomplexes and it coat was conduce without organic solvents, can be considered a green process, and allowed to obtain water soluble particles. Furthermore, CNC covering the nanocomplexes brought additional protection to the system can contribut advancement in the pharmaceutical. (AU)

• Processo FAPESP: 19/22626-5 - Sistema biomimético estruturado a partir da gelificação ionotrópica com a adsorção de nanocristais de celulose bacteriana
• Beneficiário: Victória Soares Soeiro
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 18/10508-5 - Produção de nanocristais de celulose bacteriana para estabilização de nanoemulsões e transporte transepitelial de fármacos
• Beneficiário: Victória Soares Soeiro
• Modalidade de apoio: Bolsas no Brasil - Mestrado