Hydrogen and organic acid production from dark fermentation of sugarcane vinasse without buffers in mesophilic and thermophilic conditions

Autor(es)

Ribeiro, Jaqueline Cardoso ^[1] ; Mota, Vera Taina ^[2] ; de Oliveira, Valeria Maia ^[2] ; Dacanal, Gustavo Cesar ^[3] ; Zaiat, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo LPB EESC USP, Escola Engn Sao Carlos, Lab Proc Biol, Av Joao Dagnone 1100, BR-13563120 Sao Carlos, SP - Brazil

^[2] Univ Estadual Campinas CPQBA Unicamp, Div Recursos Microbianos, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, Paulinia - Brazil

^[3] Univ Sao Paulo FZEA USP, Fac Zootecnia & Engn Alimentos, Pirassununga - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY; DEC 2021.
• Citações Web of Science: 0

Resumo

BACKGROUND Sugarcane vinasse has a low pH and several studies report that alkalinity supplementation is essential to the stability of its dark fermentation (DF). In the context of biorefineries, the reduction, or even elimination, of alkalinizing agents could be extremely environmentally and economically advantageous. The main objective of this study was to evaluate the valorisation of vinasse DF through bioH(2) and value-added metabolite production without buffers in two similar Upflow Anaerobic Sludge Blanket (UASB) under mesophilic (U30) and thermophilic (U55) conditions. RESULTS Average organic acid yields of 376 +/- 52 and 248 +/- 122 mg-CODOA.g(-1)CODt(in), and productivities of 17 396 +/- 5220 and 14 024 +/- 1642 mg-CODOA.L-1 day(-1) were obtained for U30 and U55 reactors, respectively, with only vinasse as substrate (CODOA stands for organic acids chemical oxygen demand (COD) and CODin influent COD). BioH(2) production was feasible with sucrose as substrate and pH below 3.0, but it ceased with substrate replacement by vinasse along with a natural pH increase. A change in microbial community was also observed: Ethanoligenens, Clostridium sensu stricto 12, and Liquorilactobacillus (using sucrose and with a pH <3.0) were replaced by Prevotella, Megasphaera, Pectinatus, Clostridium sensu stricto 11, and Lactobacillus using vinasse (with a pH > 4.0). CONCLUSION Results showed that DF of sugarcane vinasse without any buffering addition might be a promising approach for the valorization of real wastewater through the production of organic acids. Nevertheless, bioH(2) production was not favored and further strategies would be needed to maintain hydrogen-producing bacteria when sucrose is replaced by real effluent with a natural pH increase. (c) 2021 Society of Chemical Industry (SCI). (AU)

• Processo FAPESP: 15/06246-7 - Aplicação do conceito de biorrefinaria a estações de tratamento biológico de águas residuárias: o controle da poluição ambiental aliado à recuperação de matéria e energia
• Beneficiário: Marcelo Zaiat
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/25645-8 - Abordagens ômicas de reatores acidogênicos sem alcalinizantes para produção de ácidos orgânicos e hidrogênio
• Beneficiário: Vera Tainá Franco Vidal Mota
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/06940-6 - Avaliação de reatores anaeróbios acidogênicos e metanogênicos no tratamento de vinhaça e soro de queijo sem alcalinazação
• Beneficiário: Jaqueline Cardoso Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado