Evaluating the presence of Mycoplasma hyorhinis, Fusobacterium nucleatum, and Helicobacter pylori in biopsies of patients with gastric cancer

Autor(es)

do Nascimento Araujo, Camila ^[1] ; Amorim, Aline Teixeira ^[1] ; Barbosa, Maysa Santos ^[1] ; Alexandre, Julieta Canjimba Porto Lucas ^[2] ; Campos, Guilherme Barreto ^{[1, 3]} ; Macedo, Claudia Leal ^[4] ; Marques, Lucas Miranda ^{[1, 3]} ; Timenetsky, Jorge ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, ICB USP, Dept Microbiol, Sao Paulo - Brazil

^[2] Jose Eduardo Santos Univ, Dept Anim Hlth, Fac Vet Med, Huambo - Angola

^[3] Univ Fed Bahia, Multidisciplinary Hlth Inst, IMS CAT UFBA, Campus Anisio Teixeira, Vitoria - Brazil

^[4] Micro Pathol Anat & Cytopathol Serv, Vitoria Da Conquista - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INFECTIOUS AGENTS AND CANCER; v. 16, n. 1 DEC 23 2021.
• Citações Web of Science: 0

Resumo

Background Gastric cancer is the third leading cause of cancer-related deaths worldwide and has been associated with infections that may promote tumour progression. Accordingly, we analysed the presence of Mollicutes, Mycoplasma hyorhinis, Fusobacterium nucleatum and Helicobacter pylori in gastric cancer tissues and evaluated their correlation with clinicopathological factors. Methods Using a commercial kit, DNA were extracted from 120 gastric samples embedded in paraffin: 80 from patients with gastric cancer and 40 from cancer free patients, dating from 2006 to 2016. Mollicutes and H. pylori were detected by PCR; F. nucleatum and M. hyorhinis were detected by qPCR, together with immunohistochemistry for the latter bacteria. Results Mollicutes were detected in the case and control groups (12% and 2.5%) and correlated with the papillary histologic pattern (P = 0.003), likely due to cell transformation promoted by Mollicutes. M. hyorhinis was detected in the case and control group but was not considered a cancer risk factor. H. pylori was detected at higher loads in the case compared to the control group (8% and 22%, P = 0.008) and correlated with metastasis (P = 0.024), lymphatic invasion (P = 0.033), tumour of diffused type (P = 0.028), and histopathological grading G1/G2 (P = 0.008). F. nucleatum was the most abundant bacteria in the case group, but was also detected in the control group (26% and 2.5%). It increased the cancer risk factor (P = 0.045, OR = 10.562, CI95% = 1.057-105.521), and correlated with old age (P = 0.030) and tumour size (P = 0.053). Bacterial abundance was significantly different between groups (P = 0.001). Conclusion Our findings could improve the control and promote our understanding of opportunistic bacteria and their relevance to malignant phenotypes. (AU)

• Processo FAPESP: 14/05071-6 - Avaliação da participação dos Mollicutes e outros microrganismos de interesse genital na endometriose humana
• Beneficiário: Jorge Timenetsky
• Modalidade de apoio: Auxílio à Pesquisa - Regular