Intercellular interactions between mast cells and stromal fibroblasts obtained from canine cutaneous mast cell tumours

Autor(es)

Pulz, Lidia H. ^{[1, 2]} ; Cordeiro, Yonara G. ^[2] ; Huete, Greice C. ^[2] ; Cadrobbi, Karine G. ^[2] ; Rochetti, Arina L. ^[2] ; Xavier, Pedro L. P. ^[2] ; Nishiya, Adriana Tomoko ^[3] ; de Freitas, Silvio Henrique ^[2] ; Fukumasu, Heidge ^[2] ; Strefezzi, Ricardo F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Lab Oncol Comparada & Translat, Campus Fernando Costa, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[3] Univ Anhembi Morumbi, Hosp Vet, R Conselheiro Lafaiete 64, BR-0310100 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 DEC 13 2021.
• Citações Web of Science: 0

Resumo

Mast cell tumours (MCTs) are the most frequent malignant skin neoplasm in dogs. Due to the difficulty in purifying large numbers of canine neoplastic mast cells, relatively little is known about their properties. A reproducible in vitro model is needed to increase the understanding about the phenotype and functional properties of neoplastic mast cells. In the present study, we describe the establishment of primary cocultures of neoplastic mast cells from canine cutaneous MCTs and cancer-associated fibroblasts. We confirmed the inability of canine neoplastic mast cells to remain viable for long periods in vitro without the addition of growth factors or in vivo passages in mice. Using a transwell system, we observed that mast cell viability was significantly higher when there is cell-to-cell contact in comparison to non-physical contact conditions and that mast cell viability was significantly higher in high-grade than in low-grade derived primary cultures. Moreover, the use of conditioned medium from co-cultured cells led to a significantly higher tumoral mast cell viability when in monoculture. Signalling mechanisms involved in these interactions might be attractive therapeutic targets to block canine MCT progression and deserve more in-depth investigations. (AU)

• Processo FAPESP: 21/11567-8 - Interações intercelulares entre mastócitos e fibroblastos estromais obtidos de mastocitomas cutâneos caninos
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Processo FAPESP: 16/03862-1 - Caracterização de fibroblastos associados ao câncer e sua influência sobre neoplasias caninas
• Beneficiário: Ricardo de Francisco Strefezzi
• Modalidade de apoio: Auxílio à Pesquisa - Regular