Genomic characterization and antimicrobial resistance profiles of Salmonella enterica serovar Infantis isolated from food, humans and veterinary-related sources in Brazil

Autor(es)

Vilela, Felipe Pinheiro ^[1] ; Rodrigues, Dalia dos Prazeres ^[2] ; Allard, Marc William ^[3] ; Falcao, Juliana Pfrimer ^[1]

Afiliação do(s) autor(es)

^[1] Fac Ciencias Farmaceut Ribeirao Preto USP, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, SP - Brazil

^[2] Fundacao Oswaldo Cruz Fiocruz, Rio De Janeiro, RJ - Brazil

^[3] US FDA, Div Microbiol, Off Regulatory Sci, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Microbiology; JAN 2022.
• Citações Web of Science: 0

Resumo

Aims To characterize the genetic relatedness, phenotypic and genotypic antimicrobial resistance and plasmid content of 80 Salmonella Infantis strains isolated from food, humans and veterinary sources from 2013 to 2018 in Brazil. Methods and results Pulsed-field gel electrophoresis and single-nucleotide polymorphism analysis showed major clusters containing 50% and 38.8% of the strains studied respectively. Multilocus sequence typing assigned all strains to ST32. Disk-diffusion revealed that 90% of the strains presented resistant or intermediate resistant profiles and 38.8% displayed multidrug resistance. Resistance genes for aminoglycosides (aac(6 `)-Iaa; aadA12; aph(3 `'-Ib; aph(6)-Id), beta-lactams (bla(TEM-1); bla(CTX-M-8); bla(CMY-2)), trimethoprim (dfrA8), tetracycline (tet(A)), amphenicols (floR), sulfonamide (sul2), efflux pumps (mdsA; mdsB), chromosomal point mutations in gyrB, parC, acrB and pmrA were detected. Strains harboured IncI, IncF, IncX, IncQ, IncN and IncR plasmids. Conclusions The presence of a prevalent S. Infantis subtype in Brazil and the high antimicrobial resistance rates reinforced the potential hazard of this serovar for the public health and food safety fields. Significance and Impact of the Study This is the first study characterizing a large set of S. Infantis from Brazil by whole-genome sequencing, which provided a better local and global comprehension about the distribution and characteristics of this serovar of importance in the food, human and veterinary fields. (AU)

• Processo FAPESP: 19/19338-8 - Genômica, transcriptômica e caracterização fenotipica de linhagens de Campylobacter jejuni isoladas de diversas fontes durante 20 anos no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/06947-6 - Caracterização molecular da diversidade genotípica, perfil de resistência e potencial patogênico de linhagens de Salmonella infantis isoladas de fontes diversas no Brasil
• Beneficiário: Felipe Pinheiro Vilela
• Modalidade de apoio: Bolsas no Brasil - Mestrado