MT in salivary gland tumors: the expression of microRNAs miR-155 and miR-200c is associated with clinical-pathological parameter

Autor(es)

Kerche, Leandra Ernst ^[1] ; de Sousa, Elen Alves ^[1] ; Squarize, Cristiane Helena ^{[2, 3]} ; Oliveira, Katia Klug ^[1] ; Marchi, Fabio Albuquerque ^[1] ; Bettim, Barbara Beltrame ^[1] ; Kowalski, Luiz Paulo ^[4] ; Soares, Fernando Augusto ^[5] ; Lourenco, Silvia Vanessa ^[6] ; Coutinho-Camillo, Claudia Malheiros ^[1]

Afiliação do(s) autor(es)

^[1] Ctr Int Pesquisa, AC Camargo Canc Ctr, Int Res Ctr, Rua Tagua, 440 Primeiro Andar, Sao Paulo, SP - Brazil

^[2] Univ Michigan, Dept Periodont & Oral Med, Lab Epithelial Biol, Sch Dent, Ann Arbor, MI 48109 - USA

^[3] Univ Michigan, Michigan Med Rogel Canc Ctr, Ann Arbor, MI 48109 - USA

^[4] AC Camargo Canc Ctr, Dept Head & Neck Surg & Otorhinolaryngol, Sao Paulo, SP - Brazil

^[5] Rede DOr Hosp Network, Pathol Div, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Dent Sch, Dept Gen Pathol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. 49, n. 3 JAN 2022.
• Citações Web of Science: 0

Resumo

Background Epithelial to mesenchymal transition promotes cell adhesion loss, enabling invasion and metastasis. MicroRNAs are a class of small non-codifying RNAs that regulate gene expression. Objectives The aim of this study was to evaluate the expression of microRNAs that could regulate the expression of EMT factors in salivary gland tumors (SGTs). Methods and results The expression of microRNAs miR-9, miR-34a, miR-101, miR-138, miR-155, and miR-200c-described in the literature to target EMT factors-was evaluated by Real-time RT-PCR (qPCR) in pleomorphic adenoma (PA), mucoepidermoid carcinoma (MEC) and adenoid cystic carcinoma (ACC) samples. Bioinformatics tools were applied to identify miR targets and immunohistochemistry was used to examine the expression of the proteins E-cadherin, Twist, ZEB-1, beta-Catenin, and c-Kit. Comparing miR expression among SGT types, we observed increased expression of miR-9, and miR-138 in PAs, and increased miR-155 expression in MECs. Low-grade MECs exhibited increased miR-155 expression (p = 0.032). MECs that generated lymph node metastases had increased miR-200c levels (p = 0.018). MECs tended to have decreased expression of EMT-related proteins when compared to the other SGT types (c-Kit p < 0.001, Twist p = 0.014, and ZEB p = 0.012). Notably, increased c-Kit expression was associated with the presence of perineural infiltration in ACC (p = 0.050). Conclusions This study provides evidence of alterations in the expression of EMT-factors regulating miRs, especially of miR-9, miR-138, miR-155, and miR-200c. No significant relationships were found between the expression of these miRs and proteins associated with EMT in SGTs. (AU)

• Processo FAPESP: 11/02051-6 - Apoptose nas glândulas salivares humanas: avaliação de marcadores na morfogênese e no processo de tumorigênese da glândula utilizando como modelos o adenoma pleomórfico e o carcinoma mucoepidermóide
• Beneficiário: Cláudia Malheiros Coutinho Camillo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores