Autonomic Disbalance During Systemic Inflammation is Associated with Oxidative Stress Changes in Sepsis Survivor Rats

Autor(es)

Amorim, Mateus R. ^{[1, 2]} ; de Jesus, Aline A. ^[3] ; Santos-Junior, Nilton N. ^[3] ; Rocha, Maria J. A. ^[2] ; Nogueira, Jonatas E. ^[4] ; Batalhao, Marcelo E. ^[5] ; Carnio, Evelin C. ^[5] ; Branco, Luiz G. S. ^[2]

Afiliação do(s) autor(es)

^[1] Johns Hopkins Univ, Sch Med, Dept Med, Div Pulm & Crit Care Med, Baltimore, MD - USA

^[2] Univ Sao Paulo, Dent Sch Ribeirao Preto, Ave Cafe S-N, BR-14040904 Sao Paulo, RP - Brazil

^[3] Univ Sao Paulo, Med Sch Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sports Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Nursing Sch Ribeirao Preto, BR-14040902 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Inflammation; JAN 2022.
• Citações Web of Science: 0

Resumo

Sepsis affects 31.5 million people worldwide. It is characterized by an intense drop in blood pressure driving to cardiovascular morbidity and mortality. Modern supportive care has increased survival in patients; however, after experiencing sepsis, several complications are observed, which may be potentiated by new inflammatory events. Nevertheless, the interplay between sepsis survivors and a new immune challenge in cardiovascular regulation has not been previously defined. We hypothesized that cecal ligation and puncture (CLP) cause persistent cardiovascular dysfunctions in rats as well as changes in autonomic-induced cardiovascular responses to lipopolysaccharide (LPS). Male Wistar rats had mean arterial pressure (MAP) and heart rate (HR) recorded before and after LPS or saline administration to control or CLP survivor rats. CLP survivor rats had similar baseline MAP and HR when compared to control. LPS caused a drop in MAP accompanied by tachycardia in control, while CLP survivor rats had a noteworthy enhanced MAP and a blunted tachycardia. LPS-induced hemodynamic changes were related to an autonomic disbalance to the heart and resistance vessels that were expressed as an increased low- and high-frequency power of pulse interval in CLP survivors after saline and enhancement in the low-frequency power of systolic arterial pressure in control rats after LPS. LPS-induced plasma interferon gamma, but not interleukin-10 surges, was blunted in CLP survivor rats. To further access whether or not LPS-induced autonomic disbalance in CLP survivor rats was associated with oxidative stress dysregulation, superoxide dismutase (SOD) activity and thiobarbituric acid reactive substances (TBARS) plasma levels changes were measured. LPS-induced oxidative stress was higher in CLP survivor rats. These findings indicate that key changes in hemodynamic regulation of CLP survivors rats take place in response to LPS that are associated with oxidative stress changes, i.e., reduced SOD activity and increased TBARS levels. (AU)

• Processo FAPESP: 17/09878-0 - Efeito da estimulação vagal durante desafio imune por LPS em ratos normotensos e espontaneamente hipertensos
• Beneficiário: Mateus Ramos Amorim
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/17681-9 - Alterações fisiopatológicas durante a inflamação sistêmica
• Beneficiário: Luiz Guilherme de Siqueira Branco
• Modalidade de apoio: Auxílio à Pesquisa - Temático