egree of piRNA sharing and Piwi gene expression in the skeletal muscle of Piaractus mesopotamicus (pacu), Colossoma macropomum (tambaqui), and the hybrid tambac

Autor(es)

de Paula, Tassiana Gutierrez ^[1] ; Thomazini Zanella, Bruna Tereza ^[1] ; de Moraes, Leonardo Nazario ^[1] ; Valente, Jessica Silvino ^[1] ; Nascimento-Oliveira, Jordana Inacio ^[1] ; Mareco, Edson Assuncao ^[2] ; Fernandez, Geysson Javier ^[3] ; Dal-Pai-Silva, Maeli ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, BR-18618970 Botucatu, SP - Brazil

^[2] Univ Western Sao Paulo Unoeste, Presidente Prudente, SP - Brazil

^[3] Univ Antioquia, Fac Med, Dept Inmmunovirol, Medellin - Colombia

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY; v. 264, FEB 2022.
• Citações Web of Science: 0

Resumo

PiRNAs are a class of small noncoding RNAs that, in their mature form, bind to Piwi proteins to repress transposable element activity. Besides their role in gametogenesis and genome integrity, recent evidence indicates their action in non-germinative tissues. We performed a global analysis of piRNA and Piwi gene expression in the skeletal muscle of juveniles pacu (Piaractus mesopotamicus), tambaqui (Colossoma macropomum), and the hybrid tambacu to evaluate the degree of piRNA sharing among these three genotypes. Total RNA was sequenced and analyzed using specific parameters of piRNAs by bioinformatics tools. piRNA and Piwi gene expression was analyzed by RT-qPCR. We detected 24 piRNA clusters common to the three genotypes, with eight shared between pacu and tambacu, three between pacu and tambaqui, and five between tambaqui and tambacu; seven, five, and four clusters were unique to pacu, tambacu, and tambaqui, respectively. Genomic localization and fold change values showed two clusters and 100 piRNAs shared among the three genotypes. The gene expression of four piRNAs was evaluated to validate our bioinformatics results. piRNAs from cluster 17 were higher in tambacu than pacu and piRNAs from cluster 18 were more highly expressed in tambacu than tambaqui and pacu. In addition, the expression of Piwis 1 and 2 was higher in tambacu and tambaqui than pacu. Our results open an important window to investigate whether these small noncoding RNAs benefit the hybrid in terms of faster growth and offer a new perspective on the function of piRNAs and Piwis in fish skeletal muscle. (AU)

• Processo FAPESP: 17/16479-4 - Perfil global de expressão de piRNAs no músculo esquelético de Piaractus mesopotamicus (pacu), Colossoma macropomum (tambaqui) e seu híbrido, tambacu
• Beneficiário: Tassiana Gutierrez de Paula
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/05009-4 - Análise comparativa e integrativa do transcriptoma e microRNAoma do músculo esquelético de Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu) e seu híbrido, tambacu: abordagens moleculares, in silico, in vitro e in vivo.
• Beneficiário: Maeli Dal Pai
• Modalidade de apoio: Auxílio à Pesquisa - Regular