Characterization and in vitro testing of newly isolated lytic bacteriophages for the biocontrol of Pseudomonas aeruginosa

Autor(es):Mostrar menos -

Harada, Liliam K. ^[1] ; Silva, Erica C. ^[1] ; Rossi, Fernando P. N. ^[2] ; Cieza, Basilio ^[3] ; Oliveira, Thais J. ^[1] ; Pereira, Carla ^{[4, 5]} ; Tomazetto, Geizecler ^[6] ; Silva, Bianca B. ^[1] ; Squina, Fabio M. ^[1] ; Vila, Marta M. D. C. ^[1] ; Setubal, Joao C. ^[2] ; Ha, Taekjip ^[3] ; da Silva, Aline M. ^[2] ; Balcao, Victor M. ^{[4, 5, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sorocaba, PhageLab Lab Biofilms & Bacteriophages, Sorocaba, SP - Brazil

^[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil

^[3] Johns Hopkins Univ, Dept Biophys & Biophys Chem, Baltimore, MD - USA

^[4] Univ Aveiro, Dept Biol, Campus Univ Santiago, Aveiro - Portugal

^[5] Univ Aveiro, CESAM, Campus Univ Santiago, Aveiro - Portugal

^[6] Aarhus Univ, Biol & Chem Engn Sect BCE, Dept Engn, Aarhus - Denmark

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 17, n. 2, p. 111-141, JAN 2022.
• Citações Web of Science: 0

Resumo

Aim: Two lytic phages were isolated using P. aeruginosa DSM19880 as host and fully characterized. Materials \& methods: Phages were characterized physicochemically, biologically and genomically. Results \& conclusion: Host range analysis revealed that the phages also infect some multidrug-resistant (MDR) P. aeruginosa clinical isolates. Increasing MOI from 1 to 1000 significantly increased phage efficiency and retarded bacteria regrowth, but phage ph0034 (reduction of 7.5 log CFU/ml) was more effective than phage ph0031 (reduction of 5.1 log CFU/ml) after 24 h. Both phages belong to Myoviridae family. Genome sequencing of phages ph0031 and ph0034 showed that they do not carry toxin, virulence, antibiotic resistance and integrase genes. The results obtained are highly relevant in the actual context of bacterial resistance to antibiotics. (AU)

• Processo FAPESP: 18/05522-9 - PsaPhageKill - isolamento, caracterização e uso de bacteriófagos líticos contra Pseudomonas syringae pv. actinidiae para combater o cancro do kiwi: uma alternativa eficiente e amiga do ambiente
• Beneficiário: Vitor Manuel Cardoso Figueiredo Balcão
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 16/12234-4 - TransAppIL - Permeação cutânea de entidades proteicas estabilizadas estruturalmente e funcionalmente, carreadas por líquidos iônicos.
• Beneficiário: Marta Maria Duarte Carvalho Vila
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/08884-3 - PneumoPhageColor - desenvolvimento de um kit de biodetecção colorimétrica de Pseudomonas aeruginosa com base em partículas fágicas
• Beneficiário: Vitor Manuel Cardoso Figueiredo Balcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular