Characterization and in vitro testing of newly isolated lytic bacteriophages for the biocontrol of Pseudomonas aeruginosa

Author(s):Show less -

Harada, Liliam K. ^[1] ; Silva, Erica C. ^[1] ; Rossi, Fernando P. N. ^[2] ; Cieza, Basilio ^[3] ; Oliveira, Thais J. ^[1] ; Pereira, Carla ^{[4, 5]} ; Tomazetto, Geizecler ^[6] ; Silva, Bianca B. ^[1] ; Squina, Fabio M. ^[1] ; Vila, Marta M. D. C. ^[1] ; Setubal, Joao C. ^[2] ; Ha, Taekjip ^[3] ; da Silva, Aline M. ^[2] ; Balcao, Victor M. ^{[4, 5, 1]}

Affiliation

^[1] Univ Sorocaba, PhageLab Lab Biofilms & Bacteriophages, Sorocaba, SP - Brazil

^[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil

^[3] Johns Hopkins Univ, Dept Biophys & Biophys Chem, Baltimore, MD - USA

^[4] Univ Aveiro, Dept Biol, Campus Univ Santiago, Aveiro - Portugal

^[5] Univ Aveiro, CESAM, Campus Univ Santiago, Aveiro - Portugal

^[6] Aarhus Univ, Biol & Chem Engn Sect BCE, Dept Engn, Aarhus - Denmark

• Document type: Journal article
• Source: FUTURE MICROBIOLOGY; v. 17, n. 2, p. 111-141, JAN 2022.
• Web of Science Citations: 0

Abstract

Aim: Two lytic phages were isolated using P. aeruginosa DSM19880 as host and fully characterized. Materials \& methods: Phages were characterized physicochemically, biologically and genomically. Results \& conclusion: Host range analysis revealed that the phages also infect some multidrug-resistant (MDR) P. aeruginosa clinical isolates. Increasing MOI from 1 to 1000 significantly increased phage efficiency and retarded bacteria regrowth, but phage ph0034 (reduction of 7.5 log CFU/ml) was more effective than phage ph0031 (reduction of 5.1 log CFU/ml) after 24 h. Both phages belong to Myoviridae family. Genome sequencing of phages ph0031 and ph0034 showed that they do not carry toxin, virulence, antibiotic resistance and integrase genes. The results obtained are highly relevant in the actual context of bacterial resistance to antibiotics. (AU)

• FAPESP's process: 18/05522-9 - PsaPhageKill - isolation, characterisation and use of lytic bacteriophages against Pseudomonas syringae PV. actinidiae to fight the cancer of kiwifruit: an efficient and ecofriendly alternative
• Grantee: Vitor Manuel Cardoso Figueiredo Balcão
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 16/12234-4 - TransAppIL - Transdermal delivery of structurally and functionally stabilized protein entities applying ionic liquids.
• Grantee: Marta Maria Duarte Carvalho Vila
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/08884-3 - PneumoPhageColor - development of a colorimetric biodetection kit for Pseudomonas aeruginosa based on phage particles
• Grantee: Vitor Manuel Cardoso Figueiredo Balcão
• Support Opportunities: Regular Research Grants