itamin D decreases expression of NLRP1 and NLRP3 inflammasomes in placental explants from women with preeclampsia cultured with hydrogen peroxid

Autor(es)

Nunes, Priscila R. ^[1] ; Romao-Veiga, Mariana ^[1] ; Matias, Mariana L. ^[1] ; Ribeiro, Vanessa R. ^[1] ; de Oliveira, Leandro ^[1] ; Peracoli, Jose Carlos ^[1] ; Peracoli, Maria Terezinha S. ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Inst Biosci, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HUMAN IMMUNOLOGY; v. 83, n. 1, p. 74-80, JAN 2022.
• Citações Web of Science: 0

Resumo

This study aimed to evaluate the immunomodulatory effect of vitamin D (VD) on the NLRP1 and NLRP3 inflammasomes in placental explants from preeclamptic (PE) and normotensive (NT) pregnant women. Placental explants from eight PE and eight NT pregnant women were cultured with or without hydrogen peroxide (H2O2), VD or H2O2 + VD. Gene and protein expression of NLRP1, NLRP3, HMGB1, caspase-1, IL-1 beta, TNF-alpha and IL-18 were determined by qPCR and Western blotting/ELISA. Compared to NT pregnant women, the endogenous gene expression of NLRP1, NLRP3, HMGB1, IL-1 beta, TNF-alpha and IL-18 was signifi- cantly higher in explants from PE and became decreased after VD treatment. Similarly, VD decreased the protein expression of NLRP1, NLRP3, caspase-1, HMGB1, IL-1 beta, TNF-alpha and IL-18 in PE. Placental explants from NT cultured with H2O2 showed increased gene and protein expression of NLRP1, NLRP3, caspase-1, IL-1 beta, TNF-alpha and HMGB1, while H2O2 was also able to increase TNF-alpha and caspase-1 gene expression in PE. Treatment with H2O2 + VD decreased gene/protein expression of NLRP1, NLRP3, caspase-1, HMGB1, IL-1 beta, TNF-alpha and IL-18 in PE and NT explants with H2O2. NLRP1 and NLRP3 are upregulated in the PE. VD may play an immunomodulatory role in the placental inflammation and downregulates oxidative stress induced in vitro by H2O2. (C) 2021 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 16/18155-9 - Modulação da resposta inflamatória sistêmica na pré-eclâmpsia
• Beneficiário: Maria Terezinha Serrão Peraçoli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/23452-2 - Efeito imunomodulador da vitamina D sobre a ativação de inflamassomas em tecido placentário de gestantes portadoras de pré-eclâmpsia
• Beneficiário: Priscila Rezeck Nunes
• Modalidade de apoio: Bolsas no Brasil - Doutorado