alivary Microbial Dysbiosis Is Associated With Peri-Implantitis: A Case-Control Study in a Brazilian Populatio

Autor(es):Mostrar menos -

Pallos, Debora ^[1] ; Sousa, Vanessa ^[2] ; Feres, Magda ^[3] ; Retamal-Valdes, Belen ^[3] ; Chen, Tsute ^[4] ; Curtis, Mike ^[5] ; Boaventura, Richardson Mondego ^[1] ; Tanaka, Marcia Hiromi ^[1] ; Salomao, Gustavo Vargas da Silva ^[6] ; Zanella, Louise ^[7] ; Tozetto-Mendoza, Tania Regina ^[8] ; Schwab, Gabriela ^[8] ; Franco, Lucas Augusto Moyses ^[8] ; Sabino, Ester Cerdeira ^[8] ; Braz-Silva, Paulo Henrique ^{[6, 8]} ; Shibli, Jamil Awad ^[3]

Afiliação do(s) autor(es)

^[1] Univ Santo Amaro, Dept Dent, Sao Paulo - Brazil

^[2] Queen Mary Univ London, Barts & London Sch Med & Dent, Inst Dent, Ctr Oral Clin Res, Ctr Oral Immunobiol & Regenerat, London - England

^[3] Univ Guarulhos, Dent Res Div, Dept Periodontol & Oral Implantol, Guarulhos - Brazil

^[4] Harvard Sch Dent Med, Dept Oral Med Infect & Immun, Boston, MA - USA

^[5] Kings Coll London, Guys Hosp Tower Wing, Inst Dent, London - England

^[6] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil

^[7] Univ La Frontera, Sci & Technol Bioresource Nucl Ctr Excellence Tra, Lab Integrat Biol LIBi, Temuco - Chile

^[8] Univ Sao Paulo, Inst Trop Med Sao Paulo, Sch Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 11, JAN 5 2022.
• Citações Web of Science: 0

Resumo

Background and ObjectivesThe aim of this study was to examine the salivary microbiome in healthy peri-implant sites and those with peri-implantitis. MethodsSaliva samples were collected from 21 participants with healthy peri-implant sites and 21 participants with peri-implantitis. The V4 hypervariable region of the 16S rRNA gene was sequenced using the Ion Torrent PGM System (Ion 318 (TM) Chip v2 400). The NGS analysis and composition of the salivary microbiome were determined by taxonomy assignment. Downstream bioinformatic analyses were performed in QIIME (v 1.9.1). ResultsClinical differences according to peri-implant condition status were found. Alpha diversity metrics revealed that the bacterial communities of participants with healthy peri-implant sites tended to have a richer microbial composition than individuals with peri-implantitis. In terms of beta diversity, bleeding on probing (BoP) may influence the microbial diversity. However, no clear partitioning was noted between the salivary microbiome of volunteers with healthy peri-implant sites or volunteers with peri-implantitis. The highest relative abundance of Stenotrophomonas, Enterococcus and Leuconostoc genus, and Faecalibacterium prausnitzii, Haemophilus parainfluenzae, Prevotella copri, Bacteroides vulgatus, and Bacteroides stercoris bacterial species was found in participants with peri-implantitis when compared with those with healthy peri-implant sites. ConclusionDifferences in salivary microbiome composition were observed between patients with healthy peri-implant sites and those with peri-implantitis. BoP could affect the diversity (beta diversity) of the salivary microbiome. (AU)

• Processo FAPESP: 13/08242-3 - Detecção de herpesvírus humanos em saliva e fluido gengival de pacientes renais crônicos
• Beneficiário: Débora Pallos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/07727-9 - Avaliação do ambiente imunoregulatório de lesões potencialmente malignas de boca, carcinomas de boca e orofaringe relacionados ou não ao HPV.
• Beneficiário: Paulo Henrique Braz da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular