Building an embryo: An auxin gene toolkit for zygotic and somatic embryogenesis in Brazilian pin

Autor(es)

Elbl, Paula M. ^[1] ; de Souza, Diego T. ^{[2, 1]} ; Rosado, Daniele ^[3] ; de Oliveira, Leandro F. ^[1] ; Navarro, V, Bruno ; Matioli, Sergio R. ^[4] ; Floh, Eny I. S. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Cell Biol, Rua Matao 277, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Math & Stat, Dept Comp Sci, Sao Paulo, SP - Brazil

^[3] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 - USA

^[4] Univ Sao Paulo, Inst Biosci, Dept Genet & Evolutionary Biol, Sao Paulo, SP - Brazil

^[5] Navarro, Bruno, V, Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Cell Biol, Rua Matao 277, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Gene; v. 817, APR 5 2022.
• Citações Web of Science: 0

Resumo

Many studies in the model species Arabidopsis thaliana characterized genes involved in embryo formation. However, much remains to be learned about the portfolio of genes that are involved in signal transduction and transcriptional regulation during plant embryo development in other species, particularly in an evolutionary context, especially considering that some genes involved in embryo patterning are not exclusive of land plants. This study, used a combination of domain architecture phylostratigraphy and phylogenetic reconstruction to investigate the evolutionary history of embryo patterning and auxin metabolism (EPAM) genes in Viridiplantae. This approach shed light on the co-optation of auxin metabolism and other molecular mechanisms that contributed to the radiation of land plants, and specifically to embryo formation. These results have potential to assist conservation programs, by directing the development of tools for obtaining somatic embryos. In this context, we employed this methodology with critically endangered and non-model species Araucaria angustifolia, the Brazilian pine, which is current focus of conservation efforts using somatic embryogenesis. So far, this approach had little success since somatic embryos fail to completely develop. By profiling the expression of genes that we identified as necessary for the emergence of land-plant embryos, we found striking differences between zygotic and somatic embryos that might explain the developmental arrest and be used to improve A. angustifolia somatic culture. (AU)

• Processo FAPESP: 18/08215-0 - Estudo dos processos de regulação gênica pós-transcricional na embriogênese somática em Araucaria angustifolia
• Beneficiário: Leandro Francisco de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/17541-2 - Do estresse ao embrião: mecanismos moleculares e fisiológicos associados à embriogênese somática
• Beneficiário: Eny Iochevet Segal Floh
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/25680-5 - Desenvolvimento e integração de ferramentas computacionais para a análise de transcriptoma de espécies de Piper
• Beneficiário: Diego Trindade de Souza
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico