fficacy of 6% Sodium Hypochlorite on Infectious Content of Teeth with Symptomatic Irreversible Pulpiti

Autor(es)

Arruda-Vasconcelos, Rodrigo ^[1] ; Barbosa-Ribeiro, Marlos ^{[1, 2]} ; Louzada, Lidiane M. ^[1] ; Lemos, Beatriz I. N. ^[1] ; de-Jesus-Soares, Adriana ^[1] ; Ferraz, Caio C. R. ^[1] ; Almeida, Jose F. A. ^[1] ; Marciano, Marina A. ^[1] ; Gomes, Brenda P. F. A. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Div Endodont, Dept Restorat Dent, Piracicaba Dent Sch, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Pernambuco UPE, Sch Dent, Arcoverde, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 48, n. 2, p. 179-189, FEB 2022.
• Citações Web of Science: 1

Resumo

Introduction: The objective of this study was to monitor the effects of chemomechanical preparation (CMP) performed with 6% sodium hypochlorite and calcium hydroxide-based intracanal medication (ICM) on the levels and diversity of bacteria, endotoxins (lipopolysaccharides {[}LPS]), and lipoteichoic acid (LTA) in root canals of teeth with symptomatic irreversible pulpitis. Methods: Samples were collected from 10 teeth with symptomatic irreversible pulpitis before CMP (S1), after CMP (S2), and after ICM (S3). The levels of bacteria, LPS, and LTA were assessed by using checkerboard DNA-DNA hybridization, LAL Pyrogent 5000, and enzyme-linked immunosorbent assay, respectively. Wilcoxon test, repeated-measures analysis of variance, and Tukey post hoc test were used for statistical analysis at a significance level of 5%. Results: Forty species were detected at S1. Two species were eliminated after CMP and 5 after ICM. Resistant and pain-related species were detected in the root canals. Higher levels of culturable bacteria were detected at S1. However, CMP and ICM effectively reduced the microbial load in the root canals. Higher levels of LPS and LTA were detected at S1. CMP was effective in reducing both LPS and LTA (P < .05). ICM produced additional reduction in the levels of LPS (P > .05) and LTA (P < .05). Conclusions: Chemomechanical preparation using 6% sodium hypochlorite and calcium hydroxide-based intracanal medication were effective in reducing the levels of bacteria, LPS, and LTA in teeth with vital pulp and irreversibly inflamed pulp. (AU)

• Processo FAPESP: 17/25242-8 - Monitoramento clínico, microbiológico e imunológico de pacientes submetidos à terapia endodôntica com diagnóstico de Pulpite irreversível
• Beneficiário: Rodrigo Arruda Vasconcelos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/23479-5 - Microbiomas e aspectos imunobiológicos nas infecções endodônticas
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/10755-5 - Investigação clínica de modulações epigenéticas em polpas dentais com diagnóstico de pulpite irreversível
• Beneficiário: Rodrigo Arruda Vasconcelos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 19/19300-0 - Investigação dos aspectos clínicos, microbiológicos e imunológicos de dentes com diagnóstico de lesão periodontal primária com envolvimento endodôntico secundário
• Beneficiário: Lidiane Mendes Louzada
• Modalidade de apoio: Bolsas no Brasil - Doutorado