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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

fficacy of 6% Sodium Hypochlorite on Infectious Content of Teeth with Symptomatic Irreversible Pulpiti

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Author(s):
Arruda-Vasconcelos, Rodrigo [1] ; Barbosa-Ribeiro, Marlos [1, 2] ; Louzada, Lidiane M. [1] ; Lemos, Beatriz I. N. [1] ; de-Jesus-Soares, Adriana [1] ; Ferraz, Caio C. R. [1] ; Almeida, Jose F. A. [1] ; Marciano, Marina A. [1] ; Gomes, Brenda P. F. A. [1]
Total Authors: 9
Affiliation:
[1] State Univ Campinas UNICAMP, Div Endodont, Dept Restorat Dent, Piracicaba Dent Sch, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Pernambuco UPE, Sch Dent, Arcoverde, PE - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 48, n. 2, p. 179-189, FEB 2022.
Web of Science Citations: 1
Abstract

Introduction: The objective of this study was to monitor the effects of chemomechanical preparation (CMP) performed with 6% sodium hypochlorite and calcium hydroxide-based intracanal medication (ICM) on the levels and diversity of bacteria, endotoxins (lipopolysaccharides {[}LPS]), and lipoteichoic acid (LTA) in root canals of teeth with symptomatic irreversible pulpitis. Methods: Samples were collected from 10 teeth with symptomatic irreversible pulpitis before CMP (S1), after CMP (S2), and after ICM (S3). The levels of bacteria, LPS, and LTA were assessed by using checkerboard DNA-DNA hybridization, LAL Pyrogent 5000, and enzyme-linked immunosorbent assay, respectively. Wilcoxon test, repeated-measures analysis of variance, and Tukey post hoc test were used for statistical analysis at a significance level of 5%. Results: Forty species were detected at S1. Two species were eliminated after CMP and 5 after ICM. Resistant and pain-related species were detected in the root canals. Higher levels of culturable bacteria were detected at S1. However, CMP and ICM effectively reduced the microbial load in the root canals. Higher levels of LPS and LTA were detected at S1. CMP was effective in reducing both LPS and LTA (P < .05). ICM produced additional reduction in the levels of LPS (P > .05) and LTA (P < .05). Conclusions: Chemomechanical preparation using 6% sodium hypochlorite and calcium hydroxide-based intracanal medication were effective in reducing the levels of bacteria, LPS, and LTA in teeth with vital pulp and irreversibly inflamed pulp. (AU)

FAPESP's process: 17/25242-8 - Clinical, microbiological and immunological monitoring of patients undertaken to endodontic therapy with diagnosis of irreversible Pulpitis
Grantee:Rodrigo Arruda Vasconcelos
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections
Grantee:Brenda Paula Figueiredo de Almeida Gomes
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/10755-5 - Clinical investigation of epigenetic modulations in dental pulp tissues with diagnosis of irreversible pulpitis
Grantee:Rodrigo Arruda Vasconcelos
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 19/19300-0 - Investigation of the clinical, microbiological and immunological aspects of teeth with diagnosis of primary periodontal lesions with secondary endodontic involvement
Grantee:Lidiane Mendes Louzada
Support Opportunities: Scholarships in Brazil - Doctorate