| Grant number: | 17/25242-8 |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| Start date: | October 01, 2018 |
| End date: | January 31, 2021 |
| Field of knowledge: | Health Sciences - Dentistry - Endodontics |
| Principal Investigator: | Brenda Paula Figueiredo de Almeida Gomes |
| Grantee: | Rodrigo Arruda Vasconcelos |
| Host Institution: | Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil |
| Associated research grant: | 15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections, AP.TEM |
| Associated scholarship(s): | 19/10755-5 - Clinical investigation of epigenetic modulations in dental pulp tissues with diagnosis of irreversible pulpitis, BE.EP.DR |
Abstract The Acute Irreversible Pulpitis (AIP) is a pathological condition of the dental pulp that has as main etiological factor the presence of bacterial biofilm and different Chemical Mediators of Inflammation (CMI), capable of generating exacerbated responses to the host. The present study aims to: 1) investigate the presence, establish the structure of the microbial community and quantify microorganisms (MO) in the different phases of the endodontic therapy by molecular and culture-dependent methods; 2) to monitor the effect of the chemical-mechanical preparation (CMP) and Intracanal Medication (ICM) on the levels of endotoxins (LPS), lipoteichoic acid (LTA) and levels of CMI and related to neurogenic pain of pulp origin; 3) correlate the clinical/radiographic characteristics of the patients with the MO found and with the levels of the CMI and pain; 4) analyze, where possible, the presence of Streptococcus mutans and Enterococcus faecalis through culture technique with specific media and genetic sequencing. The sample will consist of 20 teeth with diagnosis of AIP, randomly assigned into two groups according to the Auxiliary Chemical Substance (ACS) used - 2% chlorhexidine (CHX) gel (n = 10) or 6% sodium hypochlorite (NaOCl) (n = 10) - during the CMP. In addition, samples of teeth with normal pulp (control group) will be collected, which CMP will be performed using 2% CHX (n = 5) or 6% NaOCl (n = 5). The samples will be collected before CMP using Reciproc® file (S1); after CMP (S2); and after ICM (S3) based on calcium hydroxide. MO will be identified and quantified exclusively by molecular method (Nested-PCR and Checkerboard DNA-DNA hybridization) through specific probes; microbial culture (CFU/mL) and by molecular methods derived from samples obtained from the microbial culture (Gene Sequencing of the 16S rRNA gene [BigDye® Terminator v3.1 Cycle Sequencing] and Next Generation Sequencing [NGS]). The levels of CMI and related neurogenic pain, as well as the virulence factors of OM (IL-1±, IL-1², TNF-± and substance P, LTA) will be measured by the enzyme-linked immunosorbent assay Assay - ELISA) and LPS evaluated by the LAL Pyrogent 5000 turbidimetric method. Patient pain levels will be further measured qualitatively by visual scale and quantitatively by means of the electrical test. After the samples are processed, the data will be tabulated and analyzed statistically by SPSS for Windows. (AU) | |
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