Scholarship 19/19300-0 - Ferimentos e lesões, Ácido lipoteicoico - BV FAPESP
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Investigation of the clinical, microbiological and immunological aspects of teeth with diagnosis of primary periodontal lesions with secondary endodontic involvement

Grant number: 19/19300-0
Support Opportunities:Scholarships in Brazil - Doctorate
Start date until: March 01, 2020
End date until: February 29, 2024
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Brenda Paula Figueiredo de Almeida Gomes
Grantee:Lidiane Mendes Louzada
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated research grant:15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections, AP.TEM
Associated scholarship(s):21/14459-1 - Epigenetic characterization in teeth with vital pulp tissues and associated periodontal disease before and after endodontic procedures, BE.EP.DR

Abstract

The periodontium and dental pulp are structures closely related due to their embryonic, anatomical and functional origin. Endoperiodontal lesions are characterized by pathological changes with pulp and periodontal involvement in the same tooth. The present study aims to: a) investigate the microbiota of periodontal pockets and root canals of teeth with primary periodontal lesion and secondary endodontic involvement at the different stages of endodontic treatment through Nested PCR and Checkerboard DNA-DNA hybridization; b) monitor the effect of chemo-mechanical preparation and intracanal medication on periodontal pockets and root canals of teeth with primary periodontal lesion and secondary endodontic involvement on endotoxin and lipoteichoic acid levels by the LAL Pyrpgent 5000 turbidimetric method and enzymatic immunosorbent assay - ELISA, respectively; c) monitor levels of pro-inflammatory cytokines (IL-1±, IL-1² and TNF-±) and matrix metalloproteinases (MMP-2, -3, -8, -9 and -13), and substance P by ELISA. The sample will consist of 24 teeth diagnosed with primary periodontal lesion with secondary endodontic involvement, randomly divided into two groups according to the auxiliary chemical substance (AQS) used - chlorhexidine (CHX) gel 2% (n = 12) or sodium hypochlorite (NaOCl) 6% (n = 12) - during the chemo-mechanical preparation (CMP). Samples will be taken from the periodontal pocket and root canal before the CMP using Reciproc® (C1) file; after the CMP (C2); and after calcium hydroxide-based intracanal medication (IMC) (C3). MO will be identified and quantified by molecular method (Nested-PCR and Checkerboard DNA-DNA hybridization). The levels of chemical mediators of inflammation as well as the virulence factors of the microorganisms (IL-1±, IL-1², TNF-± and substance P, LTA) will be measured by Enzyme-Linked immunosorbent Assay (ELISA) and the LPS evaluated by the LAL Pyrogent 5000 turbidimetric method. The patients' pain levels will be further qualitatively measured by visual scale and quantitatively by the electrical test. After processing the samples, the data will be tabulated and statistically analyzed by SPSS for Windows. (AU)

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Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVES-SILVA, ESDRAS GABRIEL; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE MENDES; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO CEZAR RANDI; ALMEIDA, JOSE FLAVIO AFFONSO; MARCIANO, MARINA ANGELICA; STEINER-OLIVEIRA, CAROLINA; SANTOS, JOAO MIGUEL MARQUES; GOMES, BRENDA P. F. A.. Effect of antimicrobial photodynamic therapy on the reduction of bacteria and virulence factors in teeth with primary endodontic infection. Photodiagnosis and Photodynamic Therapy, v. 41, p. 8-pg., . (19/19300-0, 17/25090-3)
BRENDA PAULA FIGUEIREDO DE ALMEIDA GOMES; RODRIGO ARRUDA-VASCONCELOS; LIDIANE MENDES LOUZADA; REBECCA FIGUEIREDO DE ALMEIDA-GOMES; ADRIANA DE-JESUS-SOARES; LUCIA RACHEL FIGUEIREDO DE ALMEIDA; EVANDRO ROBERTO BALDACCI. SARS-CoV-2: A Professional and social gamechanger - Medical and dental aspects. Brazilian Dental Journal, v. 32, n. 5, p. 41-54, . (17/25242-8, 15/23479-5, 19/19300-0)
BARBOSA-RIBEIRO, MARLOS; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE M.; DOS SANTOS, DANIELLE G.; ANDREOTE, FERNANDO D.; GOMES, BRENDA P. F. A.. Microbiological analysis of endodontically treated teeth with apical periodontitis before and after endodontic retreatment. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 4, . (17/25242-8, 15/23479-5, 19/10755-5, 19/19300-0)
BARBOSA-RIBEIRO, MARLOS; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE M.; LIMA, AUGUSTO R.; MARCIANO, MARINA A.; ALMEIDA, JOSE FLAVIO A.; DE-JESUS-SOARES, ADRIANA; ZAIA, ALEXANDRE A.; FERRAZ, CAIO CEZAR R.; GOMES, BRENDA P. F. A.. Microbiological Investigation in Teeth with Persistent/Secondary Endodontic Infection in Different Stages of Root Canal Retreatment. EUROPEAN ENDODONTIC JOURNAL, v. 5, n. 3, p. 219-225, . (17/25242-8, 19/19300-0, 19/10755-5, 12/23697-4, 15/23479-5)
ARRUDA-VASCONCELOS, R.; LOUZADA, L. M.; FERES, M.; TOMSON, P. L.; COOPER, P. R.; GOMES, B. P. F. A.. Investigation of microbial profile, levels of endotoxin and lipoteichoic acid in teeth with symptomatic irreversible pulpitis: a clinical study. International Endodontic Journal, v. 54, n. 1, p. 46-60, . (15/23479-5, 19/10755-5, 17/25242-8, 19/19300-0)
ARRUDA-VASCONCELOS, RODRIGO; BARBOSA-RIBEIRO, MARLOS; LOUZADA, LIDIANE M.; LEMOS, BEATRIZ I. N.; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO C. R.; ALMEIDA, JOSE F. A.; MARCIANO, MARINA A.; GOMES, BRENDA P. F. A.. fficacy of 6% Sodium Hypochlorite on Infectious Content of Teeth with Symptomatic Irreversible Pulpiti. JOURNAL OF ENDODONTICS, v. 48, n. 2, p. 179-189, . (17/25242-8, 15/23479-5, 19/10755-5, 19/19300-0)
ARRUDA-VASCONCELOS, R.; CHIARELLI-NETO, V. M.; LOUZADA, L. M.; AVEIRO, E.; ALVES-SILVA, E. G.; DE-JESUS-SOARES, A.; FERRAZ, C. C. R.; ALMEIDA, J. F. A.; MARCIANO, M. A.; PECORARI, V. G. A.; et al. Quantitative analysis of culturable bacteria, levels of endotoxins, inflammatory mediators and substance P in teeth with symptomatic irreversible pulpitis and in teeth with vital normal pulp tissues. International Endodontic Journal, v. 56, n. 7, p. 10-pg., . (21/13871-6, 15/23479-5, 19/10755-5, 17/25242-8, 19/19300-0)
CHIARELLI-NETO, VITO M.; DE AVEIRO, EMELLY; BRONZATO, JULIANA D.; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE M.; GODOI, EDERALDO P.; LOPES, ERICA M.; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO C. R.; ALMEIDA, JOSE F. A.; et al. Clinical evaluation of culturable bacteria, endotoxins and lipoteichoic acid in teeth with vital normal pulp tissues. AUSTRALIAN ENDODONTIC JOURNAL, v. N/A, p. 9-pg., . (21/13871-6, 15/23479-5, 19/14448-0, 21/14570-0, 17/25242-8, 17/25090-3, 19/19300-0)
GOMES, BRENDA P. F. A.; LOUZADA, LIDIANE M.; ALMEIDA-GOMES, REBECCA F.; PINHEIRO, ERICKA T.; SOUSA, EZILMARA L. R.; JACINTO, ROGERIO C.; ARRUDA-VASCONCELOS, RODRIGO. Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study. ARCHIVES OF ORAL BIOLOGY, v. 118, . (19/19300-0, 17/25242-8, 15/23479-5)
ALVES-SILVA, ESDRAS GABRIEL; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE MENDES; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO CEZAR RANDI; ALMEIDA, JOSE FLAVIO AFFONSO; MARCIANO, MARINA ANGELICA; STEINER-OLIVEIRA, CAROLINA; BELLO-SILVA, MARINA STELLA; SHEMESH, HAGAY; et al. The effect of photodynamic therapy on postoperative pain in teeth with primary endodontic infection. Photodiagnosis and Photodynamic Therapy, v. 37, . (19/19300-0, 17/25242-8, 19/10755-5, 15/23479-5, 19/09115-1)
GOMES, BRENDA P. F. A.; BERBER, VANESSA B.; MARINHO, ARIANE C. S.; LOUZADA, LIDIANE M.; ARRUDA-VASCONCELOS, RODRIGO; PASSINI, MAICON R. Z.; LOPES, ERICA M.; PECORARI, VANESSA G. A.; CHEN, TSUTE; PASTER, BRUCE J.. Chemomechanical preparation influences the microbial community and the levels of LPS, LTA and cytokines in combined endodontic-periodontal lesions: A clinical study. JOURNAL OF PERIODONTAL RESEARCH, . (17/25242-8, 14/01655-3, 19/10755-5, 15/23479-5, 19/19300-0)

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