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Effect of chemomechanical preparation associated with photodynamic therapy in the reduction of microbial, LPS, LTA, substance p and pro-inflammatory cytokines in primary endodontic infection

Grant number: 19/09115-1
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): July 01, 2020
Effective date (End): February 28, 2021
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Brenda Paula Figueiredo de Almeida Gomes
Grantee:Esdras Gabriel Alves e Silva
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated research grant:15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections, AP.TEM


Lipopolysaccharide (LPS) or endotoxin is the main virulence factor of Gram-negative bacteria and is able to stimulate cells to produce pro-inflammatory cytokines involved in periapical tissue destruction, while lipoteichoic acid (ATL) is one of the virulence factors of Gram-positive bacteria. Photodynamic therapy (PDT) is a complementary method of disinfecting the root canal (RC), in addition to being used to treat several types of pain. Thus, the objectives of this work are: a) to study the microbiota of the RCs before and after the chemo-mechanical preparation (CMP) (experimental and control groups),after PDT (experimental group), 07 days after CMP by the Checkerboard method; b) to monitor the effect of CMP(experimental and control group) and PDT (experimental group) on cultivable bacteria levels by counting colony-forming units (CFU / mL); c) to evaluate the levels of LPS, LTA, substance P and pro-inflammatory cytokines IL1-² and TNF-± from the RCs before and after CMP (experimental and control groups), after PDT (experimental group) and 07 days after CMP (experimental ad control groups); d) to correlate clinical findings with microbiological content, levels of LPS, LTA, substance P and pro-inflammatory cytokines IL1-² and TNF-±; e) to monitor the reduction of a possible postoperative pain with and without the use of PDT. Twenty patients with teeth with necrotic pulp and periapical lesion will be selected, randomly divided into two groups: control group (n =10) conventional endodontic treatment without PDT, and the experimental group (PDT, n =10), where after CMP, PDT will be performed. The samples from CRs will be collected with sterile/pyrogenic paper cones and then will be frozen for analysis. The quantification of LPS will be performed using the LAL method; while the LTA, substance P and pro-inflammatory cytokines IL1-² and TNF-± by ELISA. All the results obtained, as well as the clinical aspects of the teeth involved, will be tabulated in Excel and later analyzed statistically by specific software. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVES-SILVA, ESDRAS GABRIEL; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE MENDES; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO CEZAR RANDI; ALMEIDA, JOSE FLAVIO AFFONSO; MARCIANO, MARINA ANGELICA; STEINER-OLIVEIRA, CAROLINA; BELLO-SILVA, MARINA STELLA; SHEMESH, HAGAY; et al. The effect of photodynamic therapy on postoperative pain in teeth with primary endodontic infection. Photodiagnosis and Photodynamic Therapy, v. 37, . (19/19300-0, 17/25242-8, 19/10755-5, 15/23479-5, 19/09115-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Esdras Gabriel Alves e. Effect chemical-mechanical preparation associated with photodynamic therapy in the reduction of microbial loard, LPS, LTA, cytokines pro-inflammatory and substance P in primary endodontic infections. 2021. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

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