Physical adsorption used to the immobilization of Burkholderia cepacia lipase into powder polymeric supports

Autor(es)

Correa, Wellington Moreira ^[1] ; Bortolozo, Ausdinir Danilo ^{[1, 2]} ; Osorio, Wislei Riuper ^{[1, 2]} ; Padilha, Giovana da Silva ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Sch Appl Sci FCA, Res Grp Mfg Adv Mat, Campus Limeira, 1300 Pedro Zaccaria St, BR-13484350 Limeira, SP - Brazil

^[2] Univ Campinas UNICAMP, Sch Technol, Campus Limeira, BR-13484332 Limeira, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY; v. 147, n. 4, p. 3071-3081, FEB 2022.
• Citações Web of Science: 0

Resumo

This study aims to combine distinctive supports with a feasible, easy and cleaning method to lipase immobilization. Commercial lipase from Burkholderia cepacia is physically adsorbed using polymeric powders such as polyhydroxybutyrate (PHB), polyhydroxybutyrate-co-hydroxyvalerate (PHBV) and copolymer styrene-divinylbenzene (St-DVB). It is found that the immobilization on PHB has a high amount of the lipase per support mass (1065 U g(-1)). The activation energy also shows a value higher than the lipase immobilized onto both PHBV (348 U g(-1)) and St-DVB (355 U g(-1)). FTIR, TG, DSC and XRD techniques are used to polymer powders and immobilized lipase characterization. Morphological arrays, loss masses, enthalpy values and crystallinity degrees are evaluated. In order to analyze the morphological aspects of the raw polymers after alcohol treatment (before immobilization) and the immobilized lipase, the optical microscopy analysis is used. The immobilized Burkholderia cepacia lipase provides distinctive behavior in the polymer powders. (AU)

• Processo FAPESP: 16/13352-0 - Influência do aquecimento por micro-ondas na correlação microestrutura-propriedades dos compostos pertencentes a família MAX.
• Beneficiário: Ausdinir Danilo Bortolozo
• Modalidade de apoio: Auxílio à Pesquisa - Regular