Physical adsorption used to the immobilization of Burkholderia cepacia lipase into powder polymeric supports

Author(s)

Correa, Wellington Moreira ^[1] ; Bortolozo, Ausdinir Danilo ^{[1, 2]} ; Osorio, Wislei Riuper ^{[1, 2]} ; Padilha, Giovana da Silva ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Sch Appl Sci FCA, Res Grp Mfg Adv Mat, Campus Limeira, 1300 Pedro Zaccaria St, BR-13484350 Limeira, SP - Brazil

^[2] Univ Campinas UNICAMP, Sch Technol, Campus Limeira, BR-13484332 Limeira, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY; v. 147, n. 4, p. 3071-3081, FEB 2022.
• Web of Science Citations: 0

Abstract

This study aims to combine distinctive supports with a feasible, easy and cleaning method to lipase immobilization. Commercial lipase from Burkholderia cepacia is physically adsorbed using polymeric powders such as polyhydroxybutyrate (PHB), polyhydroxybutyrate-co-hydroxyvalerate (PHBV) and copolymer styrene-divinylbenzene (St-DVB). It is found that the immobilization on PHB has a high amount of the lipase per support mass (1065 U g(-1)). The activation energy also shows a value higher than the lipase immobilized onto both PHBV (348 U g(-1)) and St-DVB (355 U g(-1)). FTIR, TG, DSC and XRD techniques are used to polymer powders and immobilized lipase characterization. Morphological arrays, loss masses, enthalpy values and crystallinity degrees are evaluated. In order to analyze the morphological aspects of the raw polymers after alcohol treatment (before immobilization) and the immobilized lipase, the optical microscopy analysis is used. The immobilized Burkholderia cepacia lipase provides distinctive behavior in the polymer powders. (AU)

• FAPESP's process: 16/13352-0 - Microwave heating effects on microstructure-properties correlations of the MAX compounds
• Grantee: Ausdinir Danilo Bortolozo
• Support Opportunities: Regular Research Grants