Dose- and time-dependent effects of taxifolin on viability and mineralization markers of osteoblast-like cells

Autor(es)

Massunari, Loiane ^[1] ; Rabelo, Rafaela Laruzo ^[1] ; Leite, Maria Luisa ^[2] ; Soares, Diana Gabriela ^[3] ; Anovazzi, Giovana ^[2] ; Souza Costa, Carlos Alberto ^[2] ; Duque, Cristiane ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Prevent & Restorat Dent, Aracatuba, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Oral Research; v. 35, 2021.
• Citações Web of Science: 0

Resumo

Abstract The current study evaluated the effects of taxifolin treatments on the viability of osteoblast-like cells, and on the expression of early mineralization markers, as part of the ongoing search for new endodontic materials able to induce periapical healing without causing cytotoxicity. Saos-2 osteoblast-like cells were exposed to different concentrations of taxifolin (5 and 10 µM), applied as pretreatments either for 24h and 72h, or continuously throughout the experimental protocol. Cell viability using the methylthiazole tetrazolium (MTT) assay, alkaline phosphatase activity using thymolphthalein monophosphate assays, deposition of mineralized nodules using alizarin red staining, and expression of ALP and COL-1 by qPCR were determined after 6 and 13 days of treatment. The data were analyzed statistically (p<0.05). Taxifolin was not cytotoxic in the concentrations tested. Pretreatments with taxifolin for 24h and 72h at 10 µM stimulated ALP activity, and increased mineralized nodule deposition by Saos-2 cells. Continuous treatment with taxifolin was not effective in stimulating ALP activity and mineralization. ALP and COL-1 gene expression increased with taxifolin pretreatments, since the highest mRNA levels were observed after 72h of pretreatment with taxifolin at 10 µM on day 13. In conclusion, taxifolin was cytocompatible, and induced mineralization markers when applied for short periods in osteoblast-like cell culture. (AU)

• Processo FAPESP: 15/00812-0 - Efeito de flavonóides sobre microrganismos de interesse endodôntico e sua influência na expressão de marcadores de mineralização
• Beneficiário: Loiane Massunari
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/10940-1 - Combinações de flavonóides em sistemas de hidrogéis termossensíveis como propostas de medicação endodôntica para dentes permanentes jovens
• Beneficiário: Cristiane Duque
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/02129-7 - Citotoxicidade e efeito indutor de mineralização de flavonóides sobre células osteoblásticas
• Beneficiário: Rafaela Laruzo Rabelo
• Modalidade de apoio: Bolsas no Brasil - Mestrado