| Texto completo | |
| Autor(es): |
Ferreira, Andre Lopes
[1, 2, 3, 4, 5, 6]
;
de Lima, Lucas Felipe
[1, 2, 3, 4, 5, 6]
;
Torossian Torres, Marcelo Der
[1, 2, 3, 4, 5]
;
de Araujo, William Reis
[6]
;
de la Fuente-Nunez, Cesar
[1, 2, 3, 4, 5]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Penn, Sch Engn & Appl Sci, Dept Bioengn, Philadelphia, PA 19104 - USA
[2] Univ Penn, Sch Engn & Appl Sci, Dept Chem & Biomol Engn, Philadelphia, PA 19104 - USA
[3] Univ Penn, Penn Inst Computat Sci, Philadelphia, PA 19104 - USA
[4] Univ Penn, Perelman Sch Med, Inst Translat Med & Therapeut, Machine Biol Grp, Inst Biomed Informat, Dept Psychiat, Philadelphia, PA 19104 - USA
[5] Univ Penn, Perelman Sch Med, Inst Translat Med & Therapeut, Machine Biol Grp, Inst Biomed Informat, Dept Microbiol, Philadelphia, PA 19104 - USA
[6] State Univ Campinas UNICAMP, Portable Chem Sensors Lab, Dept Analyt Chem, Inst Chem, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACS NANO; v. 15, n. 11, p. 17453-17462, NOV 23 2021. |
| Citações Web of Science: | 1 |
| Resumo | |
The COVID-19 pandemic has exacerbated our society's tremendous health equity gap. Disadvantaged populations have been disproportionally affected by COVID-19, lacking access to affordable testing, a known effective tool for preventing viral spread, hospitalizations, and deaths. Here, we describe COVID-19 Low-cost Optodiagnostic for Rapid testing (COLOR), a colorimetric biosensor fabricated on cotton swabs using gold nanoparticles modified with human angiotensin-converting enzyme 2 (ACE2), which costs 15 phi to produce and detects SARS-CoV-2 within 5 min. COLOR detected very low viral particle loads (limit of detection: 0.154 pg mL(-1) of SARS-CoV-2 spike protein), and its color intensity correlated with the cycle threshold (Ct) values obtained using reverse transcription polymerase chain reaction (RT-PCR). The performance of COLOR was assessed using 100 nasopharyngeal/oropharyngeal (NP/OP) clinical samples, yielding sensitivity, specificity, and accuracy values of 96%, 84%, and 90%, respectively. In summary, each COLOR test can be manufactured for 15 phi and presents rapid minute-time scale detection of SARS-CoV-2, thus providing a solution to enable high-frequency testing, particularly in low-resource communities. (AU) | |
| Processo FAPESP: | 18/08782-1 - Sensores químicos miniaturizados e integrados: novas plataformas de fabricação para aplicações biológicas, clínicas e ambientais |
| Beneficiário: | Mauro Bertotti |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |