ow-Cost Optodiagnostic for Minute-Time Scale Detection of SARS-CoV-

Author(s)

Ferreira, Andre Lopes ^{[1, 2, 3, 4, 5, 6]} ; de Lima, Lucas Felipe ^{[1, 2, 3, 4, 5, 6]} ; Torossian Torres, Marcelo Der ^{[1, 2, 3, 4, 5]} ; de Araujo, William Reis ^[6] ; de la Fuente-Nunez, Cesar ^{[1, 2, 3, 4, 5]}

Affiliation

^[1] Univ Penn, Sch Engn & Appl Sci, Dept Bioengn, Philadelphia, PA 19104 - USA

^[2] Univ Penn, Sch Engn & Appl Sci, Dept Chem & Biomol Engn, Philadelphia, PA 19104 - USA

^[3] Univ Penn, Penn Inst Computat Sci, Philadelphia, PA 19104 - USA

^[4] Univ Penn, Perelman Sch Med, Inst Translat Med & Therapeut, Machine Biol Grp, Inst Biomed Informat, Dept Psychiat, Philadelphia, PA 19104 - USA

^[5] Univ Penn, Perelman Sch Med, Inst Translat Med & Therapeut, Machine Biol Grp, Inst Biomed Informat, Dept Microbiol, Philadelphia, PA 19104 - USA

^[6] State Univ Campinas UNICAMP, Portable Chem Sensors Lab, Dept Analyt Chem, Inst Chem, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: ACS NANO; v. 15, n. 11, p. 17453-17462, NOV 23 2021.
• Web of Science Citations: 1

Abstract

The COVID-19 pandemic has exacerbated our society's tremendous health equity gap. Disadvantaged populations have been disproportionally affected by COVID-19, lacking access to affordable testing, a known effective tool for preventing viral spread, hospitalizations, and deaths. Here, we describe COVID-19 Low-cost Optodiagnostic for Rapid testing (COLOR), a colorimetric biosensor fabricated on cotton swabs using gold nanoparticles modified with human angiotensin-converting enzyme 2 (ACE2), which costs 15 phi to produce and detects SARS-CoV-2 within 5 min. COLOR detected very low viral particle loads (limit of detection: 0.154 pg mL(-1) of SARS-CoV-2 spike protein), and its color intensity correlated with the cycle threshold (Ct) values obtained using reverse transcription polymerase chain reaction (RT-PCR). The performance of COLOR was assessed using 100 nasopharyngeal/oropharyngeal (NP/OP) clinical samples, yielding sensitivity, specificity, and accuracy values of 96%, 84%, and 90%, respectively. In summary, each COLOR test can be manufactured for 15 phi and presents rapid minute-time scale detection of SARS-CoV-2, thus providing a solution to enable high-frequency testing, particularly in low-resource communities. (AU)

• FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
• Grantee: Mauro Bertotti
• Support Opportunities: Research Projects - Thematic Grants