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ypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profil

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Autor(es):
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Saldanha, Luiz Leonardo [1, 2, 3] ; Delgado, Aislan Quintiliano [1] ; Marcourt, Laurence [2, 3] ; de Paula Camaforte, Nathalia Aparecida [1] ; Ponce Vareda, Priscilla Maria [1] ; Ebrahimi, Samad Nejad [4] ; Vilegas, Wagner [5] ; Dokkedal, Anne Ligia [1] ; Queiroz, Emerson Ferreira [2, 3] ; Wolfender, Jean-Luc [2, 3] ; Bosqueiro, Jose Roberto [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Fac Sci, Bauru, SP - Brazil
[2] Univ Geneva, Sch Pharmaceut Sci, Geneva - Switzerland
[3] Univ Geneva, Inst Pharmaceut Sci Western Switzerland ISPSO, Geneva - Switzerland
[4] Shahid Beheshti Univ, Med Plants & Drugs Res Inst, Dept Phytochem, GC, Tehran - Iran
[5] Sao Paulo State Univ UNESP, Inst Biociences, Sao Vicente, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 16, n. 9 SEP 24 2021.
Citações Web of Science: 0
Resumo

Bauhinia holophylla leaves, also known as ``pata-de-vaca{''}, are traditionally used in Brazil to treat diabetes. Although the hypoglycemic activity of this medicinal plant has already been described, the active compounds responsible for the hypoglycemic activity have not yet been identified. To rapidly obtain two fractions in large amounts compatible with further in vivo assay, the hydroalcoholic extract of B. holophylla leaves was fractionated by Vacuum Liquid Chromatography and then purified by medium pressure liquid chromatography combined with an in vivo Glucose Tolerance Test in diabetic mice. This approach resulted in the identification of eleven compounds (1-11), including an original non-cyanogenic cyanoglucoside derivative. The structures of the isolated compounds were elucidated by nuclear magnetic resonance and high-resolution mass spectrometry. One of the major compounds of the leaves, lithospermoside (3), exhibited strong hypoglycemic activity in diabetic mice at the doses of 10 and 20 mg/kg b.w. and prevents body weight loss. The proton nuclear magnetic resonance (H-1 NMR) quantification revealed that the hydroalcoholic leaves extract contained 1.7% of lithospermoside (3) and 3.1% of flavonoids. The NMR analysis also revealed the presence of a high amount of pinitol (4) (9.5%), a known compound possessing in vivo hypoglycemic activity. The hypoglycemic properties of the hydroalcoholic leaves extract and the traditional water infusion extracts of the leaves of B. holophylla seem thus to be the result of the activity of three unrelated classes of compounds. Such results support to some extent the traditional use of Bauhinia holophylla to treat diabetes. (AU)

Processo FAPESP: 16/18628-4 - Litospermosídeo, um cianoglicosídeo não cianogênico obtido das folhas de Bauhinia holophylla (Bong.) Steud. com potente efeito hipoglicemiante: estudo dos mecanismos de ação hepáticos.
Beneficiário:José Roberto Bosqueiro
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/52237-9 - Fitoterápicos padronizados como alvo para o tratamento de doenças crônicas
Beneficiário:Wagner Vilegas
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático