| Texto completo | |
| Autor(es): |
Oguiura, Nancy
[1]
;
Correa, Poliana Garcia
[1]
;
Rosmino, Isabella Lemos
[1]
;
de Souza, Ana Olivia
[2]
;
Pasqualoto, Kerly Fernanda Mesquita
[3]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Inst Butantan, Ecol & Evolut Lab, BR-05503900 Sao Paulo, SP - Brazil
[2] Inst Butantan, Dev & Innovat Lab, BR-05503900 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Alchemy Innovat Res & Dev, BR-05508000 Sao Paulo, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | OXIN; v. 14, n. 1 JAN 2022. |
| Citações Web of Science: | 1 |
| Resumo | |
beta-defensins are antimicrobial peptides presenting in vertebrate animals. They participate in innate immunity, but little is known about them in reptiles, including snakes. Although several beta-defensin genes were described in Brazilian snakes, their function is still unknown. The peptide sequence from these genes was deduced, and synthetic peptides (with approximately 40 amino acids and derived peptides) were tested against pathogenic bacteria and fungi using microbroth dilution assays. The linear peptides, derived from beta-defensins, were designed applying the bioisosterism strategy. The linear beta-defensins were more active against Escherichia coli, Micrococcus luteus, Citrobacter freundii, and Staphylococcus aureus. The derived peptides (7-14 mer) showed antibacterial activity against those bacteria and on Klebsiella pneumoniae. Nonetheless, they did not present activity against Candida albicans, Cryptococcus neoformans, Trychophyton rubrum, and Aspergillus fumigatus showing that the cysteine substitution to serine is deleterious to antifungal properties. Tryptophan residue showed to be necessary to improve antibacterial activity. Even though the studied snake beta-defensins do not have high antimicrobial activity, they proved to be attractive as template molecules for the development of antibiotics. (AU) | |
| Processo FAPESP: | 17/11735-2 - Atividade antibiótica e determinação da estrutura de B-defensinas de Bothrops jararaca |
| Beneficiário: | Isabella Lemos Rosmino |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 08/06524-3 - Obtenção de agentes antifúngicos a partir de extratos produzidos por fungos de manguezais: isolamento e identificação do composto ativo |
| Beneficiário: | Ana Olívia de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 15/00003-5 - Genes de toxinas de venenos e b-defensinas de serpentes |
| Beneficiário: | Nancy Oguiura |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |