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MOLECULAR DEREPLICATION OF VOLATILE OILS FROM Saururus cernuus L. AND EVALUATION OF ANTI-Trypanosoma cruzi ACTIVITY

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Autor(es):
Brito, Juliana R. ; Costa-Silva, Thais A. ; Londero, Vinicius S. ; Baldim, Joao L. ; Tempone, Andre G. ; Ferreira, Edgard A. ; Lago, Joao Henrique G.
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Química Nova; v. 45, n. 2, p. 6-pg., 2022-01-01.
Resumo

The present study reports the molecular dereplication and the evaluation of anti-Ttypanosoma cruzi activity of volatile oils from inflorescences, leaves, branches, and roots of Saururus cernuus L. (Saururaceae). Chemically, the oils showed the predominance of sesquiterpenes in inflorescences (64.04%) and branches (63.82%), with beta-sesquiphellandrene (25.50%) and (E)-caryophyllene (22.40%) corresponding to the main constituents of each oil. Furthermore, it was possible to detect safrole as the most predominant compound in the leaves oil (49.09%). On the other hand, the oil from roots was mainly composed by monoterpenes (84.60%), with limonene in higher concentration (38.41%), followed by alpha-pinene (20.19%), and camphene (14.71%). The oils from inflorescences and branches displayed higher antitrypanosomal potency with EC50 values of 7.1 and 8.8 mu g mL(-1), respectively, followed by the oils from the roots and inflorescences, with EC50 values of 17.3 and 30.4 mu g mL(-1), respectively. Additionally, branches and inflorescences oils displayed no toxicity in mammalian NCTC cells (CC50 > 200 mu g mL(-1)). Using two PLS-DA methods, it was possible to suggest that the anti-T cruzi activity of the tested oils could be associated with the presence of beta-sesquiphellandrene, safrole, beta-elemene, and alpha-zingiberene whereas threo-austrobailignan-5, beta-sesquiphellandrene, alpha-humulene, germacrene D, and bicyclogermacrene play a role in the cytotoxicity against NCTC cells. (AU)

Processo FAPESP: 21/02789-7 - Busca de metabólitos bioativos com ação antiparasitária em espécies vegetais de regiões de Mata Atlântica e Cerrado - uma abordagem química, fenotípica e metabolômica
Beneficiário:João Henrique Ghilardi Lago
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
Processo FAPESP: 18/10279-6 - Seleção e Otimização de Novos Candidatos a Fármacos para Leishmaniose e Doença de Chagas
Beneficiário:André Gustavo Tempone Cardoso
Modalidade de apoio: Auxílio à Pesquisa - Regular