Genomic analyses of ciprofloxacin-resistant Neisseria gonorrhoeae isolates recovered from the largest South American metropolitan area

Autor(es):Mostrar menos -

Cassu-Corsi, Dandara ; Santos, Fernanda F. ; Cayo, Rodrigo ; Martins, Willames M. B. S. ; Nodari, Carolina S. ; Almeida, Luiz G. P. ; Martins, Rafael A. ; Carvalho da Silva, Roberto J. ; Vasconcelos, Ana Tereza R. ; Pignatari, Antonio C. C. ; Gales, Ana C.

• Tipo de documento: Artigo Científico
• Fonte: Genomics; v. 114, n. 4, p. 6-pg., 2022-07-01.

Resumo

We sequenced 13 Neisseria gonorrhoeae isolates exhibiting distinct susceptibility profiles and which were recovered over 12 years in the metropolitan region of Sao Paulo, Brazil. Whole Genome Sequencing (WGS) was performed on an Illumina MiSeq (TM) 2 x 300 bp paired-end reads. Bioinformatics analyses were carried out using CGE, PATRIC, and BLAST databases for manual curation of obtained genomes. Multilocus sequence typing (MLST) analysis identified seven STs, namely ST1580, ST1590, ST1901, ST1902, ST8161, ST9363, and ST15640. Moreover, a diversity of mutations was observed in MtrR/G45D-A39T, PIB/G120K-A121S, and PBP1/L421P. Mutations associated with sulfonamides (DHPS/R228S) and rifampicin (RNAP/H552N) were also detected, as well as tetracycline resistance determinants, namely rpsJ/V57M and tet(M). The results presented herein can contribute to the knowledge of N. gonorrhoeae strains circulating in Sao Paulo, Brazil. (AU)

• Processo FAPESP: 16/01889-0 - Avaliação do perfil de sensibilidade aos antimicrobianos, mecanismos de resistência e caracterização genotípica de isolados de Neisseria gonorrhoeae na cidade de São Paulo - Brasil
• Beneficiário: Antonio Carlos Campos Pignatari
• Modalidade de apoio: Auxílio à Pesquisa - Regular