Expanding the Database of Signal-Anchor-Release Domain Endolysins Through Metagenomics

Autor(es)

Pardini Gontijo, Marco Tulio ; Teles, Mateus Pereira ; Pereira Vidigal, Pedro Marcus ; Brocchi, Marcelo

• Tipo de documento: Artigo Científico
• Fonte: PROBIOTICS AND ANTIMICROBIAL PROTEINS; v. 14, n. 4, p. 10-pg., 2022-05-07.

Resumo

Endolysins are bacteriophage-derived lytic enzymes with antimicrobial activity. The action of endolysins against Gram-negative bacteria remains a challenge due to the physical protection of the outer membrane. However, recent research has demonstrated that signal-anchor-release (SAR) endolysins permeate the outer membrane of Gram-negative bacteria. This study investigates 2628 putative endolysin genes identified in 183,298 bacteriophage genomes. Previously, bioinformatic approaches resulted in a database of 66 SAR endolysins. This manuscript almost doubles the list with 53 additional SAR endolysin candidates. Forty-eight of the putative SAR endolysins described in this study contained one muramidase catalytic domain, and five included additional cell wall-binding domains at the C-terminus. For the moment, SAR domains are found in four protein families: glycoside hydrolase family 19 (GH19), glycoside hydrolase family 24 (GH24), glycoside hydrolase family 25 (GH25), and glycoside hydrolase family 108 (GH108). These SAR lysis are clustered in eight groups based on biochemical properties and domain presence/absence. Therefore, in this study, we expand the arsenal of endolysin candidates that might act against Gram-negative bacteria and develop a consult database for antimicrobial proteins derived from bacteriophages. (AU)

• Processo FAPESP: 20/09815-0 - Utilização de endolisinas fágicas junto com agentes permeabilizantes de membrana contra bactérias gram-negativas multirresistentes: uma estratégia à terapia antibacteriana
• Beneficiário: Mateus Pereira Teles
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 20/01535-9 - Genômica comparativa e atividade antimicrobiana de endolisinas fágicas recombinantes contra bactérias gram-negativas multirresistentes
• Beneficiário: Marco Túlio Pardini Gontijo
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 21/00465-0 - Genômica, patogenicidade, resistência, terapia antitumoral e vacinas baseadas em Salmonella enterica
• Beneficiário: Marcelo Brocchi
• Modalidade de apoio: Auxílio à Pesquisa - Regular