Insights into agar and secondary metabolite pathways from the genome of the red alga Gracilaria domingensis (Rhodophyta, Gracilariales)

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Nakamura-Gouvea, Natalia ; Alves-Lima, Cicero ; Benites, Luiz Felipe ; Iha, Cintia ; Maracaja-Coutinho, Vinicius ; Aliaga-Tobar, Victor ; Carneiro, Marcella ; Yokoya, Nair S. ; Marinho-Soriano, Eliane ; Graminha, Marcia A. S. ; Collen, Jonas ; Oliveira, Mariana C. ; Setubal, Joao C. ; Colepicolo, Pio

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHYCOLOGY; v. 58, n. 3, p. 18-pg., 2022-03-21.

Resumo

Gracilariales is a clade of florideophycean red macroalgae known for being the main source of agar. We present a de novo genome assembly and annotation of Gracilaria domingensis, an agarophyte alga with flattened thallus widely distributed along Central and South American Atlantic intertidal zones. In addition to structural analysis, an organizational comparison was done with other Rhodophyta genomes. The nuclear genome has 78 Mbp, with 11,437 predicted coding genes, 4,075 of which did not have hits in sequence databases. We also predicted 1,567 noncoding RNAs, distributed in 14 classes. The plastid and mitochondrion genome structures were also obtained. Genes related to agar synthesis were identified. Genes for type II galactose sulfurylases could not be found. Genes related to ascorbate synthesis were found. These results suggest an intricate connection of cell wall polysaccharide synthesis and the redox systems through the use of L-galactose in Rhodophyta. The genome of G. domingensis should be valuable to phycological and aquacultural research, as it is the first tropical and Western Atlantic red macroalgal genome to be sequenced. (AU)

• Processo FAPESP: 18/26220-0 - Genoma de Gracilaria domingensis, uma macroalga de importância econômica e ambiental
• Beneficiário: Natalia Nakamura Gouvea
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 16/06931-4 - Biodiversidade e prospecção de algas de águas tropicais e da Antártica marítima
• Beneficiário: Pio Colepicolo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 19/06983-2 - Avaliação das respostas metabólicas a estresses ambientais em Gracilaria
• Beneficiário: Cícero Alves Lima Júnior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado