Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation

Autor(es)

Ciciliato, Murilo Piologo ; de Souza, Matheus Chiaradia ; Tarran, Carolina Mendes ; Tironi de Castilho, Ana Laura ; Vieira, Ana Julia ; Rozza, Ariane Leite

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICS; v. 14, n. 4, p. 11-pg., 2022-04-01.

Resumo

Gastric ulcer is one of the most frequent gastrointestinal disorders, and there is an increasing search for natural products that can heal ulcers and avoid their recurrence. We aimed to evaluate the gastroprotective activity of vanillin, including the investigation of anti-inflammatory activity and the modulation of gene expression. Wistar rats were orally treated with vehicle, carbenoxolone, or vanillin (25, 50, or 100 mg/kg) and orally received absolute ethanol to develop gastric ulcers. We analyzed the ulcer area, conducted histological analysis, and measured the levels of the inflammatory cytokines TNF-alpha, IL-6, IL-1 beta, and IFN-gamma, and anti-inflammatory cytokine IL-10 by ELISA. We analyzed mRNA expression for NF-kappa B, TNF-alpha, and I1-10. We measured NOx levels using the Griess reaction. Our results showed similar gastroprotection for the three doses. Vanillin increased mucus production and preserved gastric mucosa integrity. The gastroprotective effect was linked to anti-inflammatory activity as a result of decreasing the levels of TNF-alpha, IL-6, IL-1 beta, and IFN-gamma and increasing IL-10 levels. Vanillin downregulated the mRNA expression of NF-kappa B and TNF-alpha, upregulated the mRNA expression of I1-10, and increased NOx levels in the stomach. The gastroprotective activity of vanillin is related to the maintenance of gastric mucus and the local inflammatory response modulation. (AU)

• Processo FAPESP: 18/11484-2 - Efeito gastroprotetor da vanilina em úlceras gástricas induzidas experimentalmente
• Beneficiário: Murilo Piologo Ciciliato
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica