Autor(es)

Rodrigues, Maira S. ; Tovo-Neto, Aldo ; Rosa, Ivana F. ; Doretto, Lucas B. ; Fallah, Hamideh P. ; Habibi, Hamid R. ; Nobrega, Rafael H.

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY; v. 10, p. 16-pg., 2022-05-12.

Resumo

In vertebrates, thyroid hormones are critical players in controlling different physiological processes such as development, growth, metabolism among others. There is evidence in mammals that thyroid hormones are also an important component of the hormonal system that controls reproduction, although studies in fish remain poorly investigated. Here, we tested this hypothesis by investigating the effects of methimazole-induced hypothyroidism on the testicular function in adult zebrafish. Treatment of fish with methimazole, in vivo, significantly altered zebrafish spermatogenesis by inhibiting cell differentiation and meiosis, as well as decreasing the relative number of spermatozoa. The observed impairment of spermatogenesis by methimazole was correlated with significant changes in transcript levels for several genes implicated in the control of reproduction. Using an in vitro approach, we also demonstrated that in addition to affecting the components of the brain-pituitary-peripheral axis, T3 (triiodothyronine) also exerts direct action on the testis. These results reinforce the hypothesis that thyroid hormones are an essential element of multifactorial control of reproduction and testicular function in zebrafish and possibly other vertebrate species. (AU)

• Processo FAPESP: 17/15793-7 - Efeitos do hipotireoidismo na função testicular de Danio rerio e relação dos hormônios tireoidianos com o hormônio folículo estimulante (Fsh)
• Beneficiário: Maira da Silva Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/07620-7 - Banco de espermatogônias tronco em peixes para conservação do genótipo de espécies ameaçadas e de interesse econômico
• Beneficiário: Rafael Henrique Nóbrega
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/15319-6 - Relação do hormônio inibidor de gonadotropina (GnIH) com os hormônios tireoidianos (HTs) no eixo hipotálamo-hipófise-periférico em zebrafish (Danio rerio)
• Beneficiário: Maira da Silva Rodrigues
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 20/03569-8 - Organóides testiculares e novas abordagens in vitro para estudar as espermatogônias tronco e a espermatogênese em peixes
• Beneficiário: Rafael Henrique Nóbrega
• Modalidade de apoio: Auxílio à Pesquisa - Regular