Apical Periodontitis Healing Following Treatment is Impacted by Root Canal Sealer Composition: An in Vivo and in Vitro Investigation

Autor(es)

Léa Assed Bezerra da Silva ^[1] ; Kléber Cortês Bonifácio ^[2] ; Fernanda Souza Liévana ^[3] ; Giovana Gonçalves Martins ^[4] ; Daniel Silva-Herzog Flores ^[5] ; Mário Roberto Leonardo ^[6] ; Raquel Assed Bezerra da Silva ^[7] ; Francisco Wanderley Garcia Paula-Silva ^[8]

Afiliação do(s) autor(es)

^[1] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[2] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[3] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[4] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[5] Autonomous University of San Luis Potosí. Faculty of Dentistry - México

^[6] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[7] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

^[8] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Dentistry - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 22, 2022-12-05.

Resumo

Abstract Objective: To evaluate the periapical healing following root canal treatment in teeth with apical periodontitis (in vivo) and the cytotoxic potential of root canal sealers in vitro. Material and Methods: Apical periodontitis was induced in 60 dogs' teeth and root canals were filled with Sealapex (40 roots), EndoREZ (40 roots), intracanal dressing (20 roots), or left untreated (20 roots). After 30 and 90 days, histopathological analyses were made. In vitro, J774.1 macrophages were stimulated with root canal sealers extracts, cytotoxicity was assessed using lactate dehydrogenase assay, and qRT-PCR was used to analyze TNF-α gene expression. Results: In vivo, smaller apical periodontitis and lower inflammatory cell infiltrate were found in teeth treated with Sealapex compared to EndoREZ. In vitro, EndoREZ was cytotoxic and induced TNF-α gene expression by macrophages differently from Sealapex. Conclusion: Sealapex allowed improved tissue repair following root canal treatment in teeth with apical periodontitis compared to EndoREZ. Synthesis of TNF-α induced by LPS was enhanced by EndoREZ, whereas Sealapex prevented pro-inflammatory gene expression. (AU)

• Processo FAPESP: 08/02776-8 - Técnicas adesivas de obturação - avaliação microscópica quantitativa da reparação apical e periapical pós-tratamento de canais radiculares de dentes de cães com lesão periapical
• Beneficiário: Mario Roberto Leonardo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/16882-7 - Osteoclastogênese e expressão de metaloproteinase da matriz-9 na reparação apical e periapical pós-tratamento de canais radiculares
• Beneficiário: Renato Petille
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica