Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode

Autor(es)

Anna Carolina Borges Pereira a Costa ^[1] ; José Chibebe Junior ^[2] ; Cristiane Aparecida Pereira ^[3] ; Ana Karina da Silva Machado ^[4] ; Milton Beltrame Junior ^[5] ; Juliana Campos Junqueira ^[6] ; Antonio Olavo Cardoso Jorge ^[7]

Afiliação do(s) autor(es)

^[1] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil

^[2] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil

^[3] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil

^[4] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil

^[5] Universidade do Vale do Paraíba. Instituto de Pesquisa e Desenvolvimento - Brasil

^[6] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil

^[7] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Oral Research; v. 24, n. 4, p. 413-418, 2010-12-00.

Resumo

The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10(6) cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p < 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log10 in the RB+L+ group and of 5.16 log10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied. (AU)

• Processo FAPESP: 09/52048-1 - Efeitos da terapia fotodinâmica e de óleos essenciais de Cymbopogon citratus (DC.) Stapf, Casearia sylvestris SW e Curcuma zedoaria Roscoe, in vitro, em biofilmes formados com Candida albicans, Staphylococcus aureus e Streptococcus mutans
• Beneficiário: Antonio Olavo Cardoso Jorge
• Modalidade de apoio: Auxílio à Pesquisa - Regular