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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode

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Author(s):
Anna Carolina Borges Pereira a Costa [1] ; José Chibebe Junior [2] ; Cristiane Aparecida Pereira [3] ; Ana Karina da Silva Machado [4] ; Milton Beltrame Junior [5] ; Juliana Campos Junqueira [6] ; Antonio Olavo Cardoso Jorge [7]
Total Authors: 7
Affiliation:
[1] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil
[2] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil
[3] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil
[4] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil
[5] Universidade do Vale do Paraíba. Instituto de Pesquisa e Desenvolvimento - Brasil
[6] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil
[7] Univ. Estadual Paulista. School of Dentistry of São José dos Campos. Department of Biosciences and Oral Diagnosis - Brasil
Total Affiliations: 7
Document type: Journal article
Source: Brazilian Oral Research; v. 24, n. 4, p. 413-418, 2010-12-00.
Abstract

The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10(6) cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p < 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log10 in the RB+L+ group and of 5.16 log10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied. (AU)

FAPESP's process: 09/52048-1 - Effects of photodynamic therapy and essential oils of the Cymbopogon citratus (DC.) Stapf, Casearia sylvestris SW and Curcuma zedoaria Roscoe, in vitro, in biofilms of Candida albicans, Staphylococcus aureus and Streptococcus mutans
Grantee:Antonio Olavo Cardoso Jorge
Support Opportunities: Regular Research Grants