Interrelated but Not Time-Aligned Response in Myogenic Regulatory Factors Demethylation and mRNA Expression after Divergent Exercise Bouts

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Telles, Guilherme Defante ; Libardi, Cleiton Augusto ; Conceicao, Miguel Soares ; Vechin, Felipe Cassaro ; Lixandrao, Manoel Emilio ; Rotea Mangone, Flavia Regina ; Pavanelli, Ana Carolina ; Nagai, Maria Aparecida ; Camera, Donny Michael ; Hawley, John A. ; Ugrinowitsch, Carlos

• Tipo de documento: Artigo Científico
• Fonte: MEDICINE AND SCIENCE IN SPORTS AND EXERCISE; v. 55, n. 2, p. 10-pg., 2023-02-01.

Resumo

IntroductionDNA methylation regulates exercise-induced changes in the skeletal muscle transcriptome. However, the specificity and the time course responses in the myogenic regulatory factors DNA methylation and mRNA expression after divergent exercise modes are unknown.PurposeThis study aimed to compare the time course changes in DNA methylation and mRNA expression for selected myogenic regulatory factors (MYOD1, MYF5, and MYF6) immediately after, 4 h after, and 8 h after a single bout of resistance exercise (RE), high-intensity interval exercise (HIIE), and concurrent exercise (CE).MethodsNine healthy but untrained males (age, 23.9 +/- 2.8 yr; body mass, 70.1 +/- 14.9 kg; peak oxygen uptake [V?O-2peak], 41.4 +/- 5.2 mL center dot kg(-1)center dot min(-1); mean +/- SD) performed a counterbalanced, randomized order of RE (4 x 8-12 repetition maximum), HIIE (12 x 1 min sprints at V?O-2peak running velocity), and CE (RE followed by HIIE). Skeletal muscle biopsies (vastus lateralis) were taken before (REST) immediately (0 h), 4 h, and 8 h after each exercise bout.ResultsCompared with REST, MYOD1, MYF5, and MYF6, mean methylation across all CpGs analyzed was reduced after 4 and 8 h in response to all exercise protocols (P < 0.05). Reduced levels of MYOD1 methylation were observed after HIIE and CE compared with RE (P < 0.05). Compared with REST, all exercise bouts increased mRNA expression over time (MYOD1 at 4 and 8 h, and MYF6 at 4 h; P < 0.05). MYF5 mRNA expression was lower after 4 h compared with 0 h and higher at 8 h compared with 4 h (P < 0.05).ConclusionsWe observed an interrelated but not time-aligned response between the exercise-induced changes in myogenic regulatory factors demethylation and mRNA expression after divergent exercise modes. Despite divergent contractile stimuli, changes in DNA methylation and mRNA expression in skeletal muscle were largely confined to the late (4-8 h) recovery period and similar between the different exercise challenges. (AU)

• Processo FAPESP: 18/12150-0 - Treinamento concorrente: efeito no mecanismo epigenético de metilação do DNA
• Beneficiário: Cleiton Augusto Libardi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/16513-0 - Efeito do treinamento concorrente no mecanismo epigenético de metilação do DNA
• Beneficiário: Guilherme Defante Telles
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 17/01297-8 - Efeito do treinamento concorrente no mecanismo epigenético de metilação do DNA
• Beneficiário: Guilherme Defante Telles
• Modalidade de apoio: Bolsas no Brasil - Mestrado