Novel bi-functional thermostable chimeric enzyme for feasible xylo-oligosaccharides production from agro-industrial wastes

Autor(es)

Martins, Manoela ; Silva, Marcos Fellipe ; Dinamarco, Taisa Magnani ; Goldbeck, Rosana

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 122, p. 10-pg., 2022-11-01.

Resumo

Arabinoxylan is a sustainable source for xylo-oligosaccharide production, as agro-industrial wastes are an abundant and the industrial interest for these prebiotics is raising due to their bioactivities for food and cos-metics. However, enzyme production costs and thermostability still represent a drawback. The main enzymes required for hydrolysis are endoxylanase and arabinofuranosidase, where GH 10 endoxylanase presents great thermostability and activity, and GH 62 enzymes are the arabinofuranosidases with the highest specificity but poor thermoresistance. In this sense, the fusion of these enzymes can generate a more stable enzyme with multiple catalytic domains in a single batch. The present study designed a bifunctional enzyme combining a GH 10 endoxylanase and a GH 62 arabinofuranosidase to enhance activity and thermostability, thus increasing the feasibility of xylo-oligosaccharides production from wastes. The chimera showed superior specificity for arabi-noxylan and halotolerance than the parental enzymes and higher efficiency than commercial cocktail. Ther-mostability improved especially for the arabinofuranosidase module, which increased 10 degrees C, enabling a process at higher temperatures and lower enzyme loads, which increases efficiency and decreases contamination. Cal-cium and manganese enhanced thermostability and activity. Xylobiose and xylotriose yields improved by 25%, and up to 7 g/L XOS was achieved from corn hull arabinoxylan hydrolysis. (AU)

• Processo FAPESP: 20/02871-2 - Proteína quimérica bifuncional de endoxilanase/arabinofuranosidase para hidrólise de resíduos agroindustriais e produção de xilo-oligossacarídeos
• Beneficiário: Manoela Martins
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/08542-3 - Biotecnologia enzimática para produção de xilo-oligossacarídeos a partir de resíduos agroindustriais lignocelulósicos visando a produção de simbióticos
• Beneficiário: Rosana Goldbeck Coelho
• Modalidade de apoio: Auxílio à Pesquisa - Regular