Conducting polymers modulating the catalytic activity of urease in thin composite films

Autor(es)

de Jesus, Cleber Gomes ; Rodrigues, Rebeca da Rocha ; Caseli, Luciano ; Peres, Laura Oliveira

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS; v. 654, p. 7-pg., 2022-09-13.

Resumo

Enzymes immobilized in matrices like conducting polymers (CPs) can form a supramolecular structure with enhanced biosensing performance, which depend on the structural polymer properties. This present paper employed CPs from the classes of thiophenes, fluorenes, phenylenes, and phenylene-vinylenes to immobilize urease as thin films and to investigate the relationship between the film structure and the enzyme activity. The films were assembled by the spin coating technique and characterized by UV-vis, fluorescence, and infrared spectroscopies. The enzymatic activity was estimated with UV-vis spectroscopy. Films containing different CPs presented specific enzyme activities dependent on polymers' size and polydispersity, but these activities were independent of their specific conductivity. Poly (9,9-dioctylfluorene-co-phenylene) (PFPh) presented the lower KM constant (1.52 mM.s(-1)) and was the polymer with lower molecular weight and polydispersity. The results suggested that small and homogeneous size chains increase the enzyme activity, which can be beneficial, generally speaking, for the construction of based-enzyme biosensors with enhanced efficiency. (AU)

• Processo FAPESP: 20/04427-2 - Influência dos polímeros conjugados na formação de biossensores para detecção de uréia
• Beneficiário: Cléber Gomes de Jesus
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/03239-0 - Interfaces nanoestruturadas para a investigação de substâncias bioativas em modelos de membrana celular e para a construção de dispositivos optoeletrônicos enzimáticos
• Beneficiário: Luciano Caseli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50869-6 - INCT 2014: em Eletrônica Orgânica INEO
• Beneficiário: Roberto Mendonça Faria
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático