| Texto completo | |
| Autor(es): Mostrar menos - |
Alves, Maria Luana
;
da Silva, Diogo Tiago
;
Pereira Spada, Julio Cesar
;
Franco Leonel, Joao Augusto
;
Benassi, Julia Cristina
;
Balbini Pereira, Nuno Wolfgang
;
Vioti, Geovanna
;
Alves-Martin, Maria Fernanda
;
de Almeida Paula, Nathalia Frigo
;
Starke-Buzetti, Wilma Aparecida
;
Ferreira de Sousa Oliveira, Tricia Maria
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Experimental Parasitology; v. 239, p. 7-pg., 2022-06-10. |
| Resumo | |
This study evaluated the humoral and cellular response in 100 cats living in an endemic area of visceral leishmaniosis (VL) using the Montenegro Skin Test (MST) and serological diagnosis and compared the MST with other diagnostic techniques. Sixty 60%, (60/100) cats were positive for MST and the diameter of positive skin reactions ranged from 5 to 9 mm. By serological methods, 74% (74/100) and 34% (34/100) had antibodies against Leishmania spp. by Immunofluorescence Antibody Test (IFAT) and Indirect Enzyme-Linked Immunosorbent Assay (ELISA), respectively. Comparing tests, the observed profiles were (1) IFAT (+)/MST (-) = 27 cats, (2) IFAT (-)/MST(+) = 13 cats, (3) IFAT(+)/MST(+) = 47 cats, (4) ELISA(+)/MST(-) = 12 cats, (5) ELISA(-)/MST(+) = 38 cats and (6) ELISA(+)/MST(+) = 22 cats. Through the combination of serological diagnosis and MST, a positivity frequency of 87% (87/100) by IFAT + MST and 72% (72/100) by ELISA + MST was identified in this cat population. Five cats (5%) were positive for Leishmania donovani complex DNA by molecular analysis, and two cats (2%) had Leishmania spp. amastigotes in lymph node smears. Therefore, the agreement between tests was classified as poor for all tests by Kappa index. The IFAT (+)/MST (+) response was the most frequent considering all cats (47%; 47/100); nonetheless, the most frequent immune expression in Polymerase Chain Reaction (PCR)-positive cats was the IFAT (+)/MST (-) profile (80%; 4/5). Five sick and PCR-positive cats, negative for Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV), that PCR sequencing matched 100% with L. donovani complex, all but one were MST negative. These results suggest that cats develop a significant cellular response against infection by parasites of the L. donovani complex, and most PCR and parasitological positive cats may be unable to develop a significant cellular response. (AU) | |
| Processo FAPESP: | 17/14855-9 - Identificação e caracterização de tripanossomatídeos que infectam gatos domésticos (Felis catus) de área endêmica para leishmaniose visceral |
| Beneficiário: | Maria Luana Alves |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 16/08018-4 - Resposta imune humoral e celular de gatos e cavalos infectados por Leishmania spp. |
| Beneficiário: | Trícia Maria Ferreira de Sousa Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |