Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates

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Ribeiro-Marques, Mariela Domiciano ; Maschio-Lima, Taiza ; Lemes, Thiago Henrique ; Siqueira, Joao Paulo Zen ; Brizzotti-Mazuchi, Natalia Seron ; Caetano, Maicon Henrique ; Almeida, Bianca Gottardo ; Mozaner, Leticia Queiroz ; Monteiro, Ruan Campos ; Camargo, Zoilo Pires ; Rodrigues, Anderson Messias ; de Almeida, Margarete Teresa Gottardo

• Tipo de documento: Artigo Científico
• Fonte: Medical Mycology; v. 61, n. 1, p. 5-pg., 2022-12-29.

Resumo

Lay Summary This study aimed to identify the species and the sexual idiomorph distribution patterns responsible for diagnosed cases of sporotrichosis in Sao Jose do Rio Preto, Brazil. We included 188 isolates of Sporothrix sp. from feline lesions and 27 of human origin. Sporotrichosis is a subcutaneous mycosis that affects animals and humans. Varying in severity, occurrences range from local lesions to systemic involvement. It is caused by thermodimorphic and saprobic fungi from the Sporothrix pathogenic clade. This study aimed to identify the species and the sexual idiomorph distribution patterns responsible for diagnosed cases of sporotrichosis in Sao Jose do Rio Preto, Brazil. We included 188 isolates of Sporothrix sp. from feline lesions and 27 of human origin, which underwent molecular identification and genotyping for mating-type MAT1-1 and MAT1-2. The results showed that Sporothrix brasiliensis is the prevalent species in feline sporotrichosis outbreaks with the overwhelming presence of a single mating-type, MAT1-2 (P <.0001), suggesting a prevalently clonal form of spread. Morphological analyses did not discriminate among cryptic species in the genus Sporothrix, and molecular identification was essential for the correct identification of the species responsible for the observed cases of sporotrichosis. Distribution analyses of MAT1-2 isolates support the hypothesis of unidirectional migration from the current epidemics in Sao Paulo and Rio de Janeiro to the municipality of Sao Jose do Rio Preto. (AU)

• Processo FAPESP: 17/27265-5 - Epidemiologia molecular e perspectivas genômicas na evolução e propagação de patógenos fúngicos emergentes
• Beneficiário: Anderson Messias Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores