Determination of selected herbicides employed in sugarcane crops by disposable pipette tip (DPX) extraction followed by LC-MS/MS and GC-MS/MS

Autor(es)

Oliveira, Thais Camarotto ; Lancas, Fernando Mauro

• Tipo de documento: Artigo Científico
• Fonte: Journal of Liquid Chromatography & Related Technologies; v. 44, n. 17-20, p. 8-pg., 2022-08-22.

Resumo

A disposable pipette tip extraction (DPX) method followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed and validated to determine eleven herbicides employed in sugarcane crops. After improving conditions, the DPX method consists of conditioning a C18 tip with 0.3 mL of methanol, followed by the same volume of water and 0.15 mL of the sample. After completing the sorption interaction, the sample was dispensed, and elution was made with 0.15 mL of methanol before collecting the extract for injection. The total extraction time was 4 min, and recoveries ranged from 59.1 to 76.5%. DPX method was compared to cartridge solid-phase extraction, which presented recoveries between 30.4 and 108.6%. SPE consumed four times more solvent, total extraction time is five times longer than DPX, and cartridges are more expensive than the DPX tips. DPX performance was also evaluated by gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The recoveries were similar to LC-MS/MS. Furthermore, the DPX-C18-LC-MS/MS method was applied to analyze sugarcane-derived juice samples. Results indicate that the method can determine the herbicides in actual samples and other sugarcane matrices. (AU)

• Processo FAPESP: 17/02147-0 - Cromatografia líquida em uma gota e seu acoplamento com espectrometria de massas: estratégias instrumentais, desenvolvimento de materiais, automatização e aplicações analíticas
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/07347-9 - Desenvolvimento de novos materiais e dispositivos para técnicas miniaturizadas de preparo de amostras na área de segurança alimentar
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/22724-7 - Novas estratégias instrumentais no desenvolvimento de métodos analíticos automatizados e miniaturizados para a determinação de compostos orgânicos mediante cromatografia líquida acoplada a espectrometria de massas
• Beneficiário: Deyber Arley Vargas Medina
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/15462-5 - Emprego do grafeno como material sorvente em técnicas miniaturizadas de preparo de amostra.
• Beneficiário: Bruno Henrique Fumes
• Modalidade de apoio: Bolsas no Brasil - Doutorado