Differential effects of estrogen receptors in the rostral ventrolateral medulla in Goldblatt hypertension

Autor(es)

Maruyama, N. O. ; Estrela, H. F. ; Sales, E. B. Oliveira ; Lucas, T. F. ; Porto, C. S. ; Bergamaschi, C. T. ; Campos, R. R.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY; v. 224, p. 8-pg., 2022-11-01.

Resumo

Previous studies have shown that 178-estradiol plays a cardioprotective role in the central nervous system (CNS) of male rats. The aim of the present study was to determine the influence of 178-estradiol on sympathetic vasomotor activity and blood pressure in a renovascular hypertensive Goldblatt two-kidney one-clip (2K-1C) male rat model. We also determined the influence of angiotensin II AT1 receptor on the expression of estrogen receptors (ER alpha, ER8, and G protein-coupled ER (GPER)) in the rostral ventrolateral medulla (RVLM) of Goldblatt rats. Experiments were performed in Goldblatt and age-matched control rats six weeks after clipping of renal artery to induce hypertension. Microinjection of 178-estradiol into the RVLM led to a greater reduction in mean arterial pressure and renal sympathetic nerve activity in controls than in 2K-1C rats. Microinjection of the GPER agonist G-1 into the RVLM led to a significantly greater increase in mean arterial pressure and renal sympathetic nerve activity in 2K-1C rats. Expression levels of estrogen receptors GPER and ER alpha, but not ER8, were signifi-cantly higher in the RVLM of 2K-1C rats than in that of the control rats. Chronic treatment with losartan significantly reduced the expression levels of estrogen receptors in the RVLM of 2K-1C rats. Taken altogether, the data suggest that the imbalance of actions between ER alpha and GPER, particularly with the predominance of GPER in the RVLM, contributes to sympathetic overactivation in male rats with Goldblatt hypertension. AT1-Angiotensin II receptor in the RVLM upregulated estrogen receptor expression in male Goldblatt rats. (AU)

• Processo FAPESP: 19/25295-0 - Comunicação entre o sistema nervoso central e rins: influências cardiovasculares, renais e autonômicas em condições fisiológicas e fisiopatológicas
• Beneficiário: Ruy Ribeiro de Campos Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático