Tempol differential effect on prostate cancer inflammation: In vitro and in vivo evaluation

Autor(es)

Rossetto, Isabela ; Santos, Felipe ; Kido, Larissa ; Lamas, Celina ; Montico, Fabio ; Cagnon, Valeria

• Tipo de documento: Artigo Científico
• Fonte: PROSTATE; v. N/A, p. 13-pg., 2022-12-22.

Resumo

BackgroundTempol is a redox-cycling nitroxide that acts directly on inflammation. However, few studies have reported the use of tempol in prostate cancer (PCa). The present study investigated the effects of tempol on inflammation related to NF-kappa B signaling, using hormone-dependent or hormone-independent cell lines and the transgenic adenocarcinoma of the mouse prostate PCa animal model in the early and late stages of cancer progression. MethodsPC-3 and LnCaP cells were exposed to different tempol doses in vitro, and cell viability assays were performed. The optimal treatment dose was chosen for subsequent analysis using western blotting. Five experimental groups were evaluated in vivo to test for tempol effects in the early (CT12 and TPL12 groups) and late stages (CT20, TPL20-I, and TLP20-II) of PCa development. The TPL groups were treated with 50 or 100 mg/kg tempol. All control groups received water as the vehicle. The ventral lobe of the prostate was collected and subjected to immunohistochemical and western blot analysis. ResultsTempol treatment reduced cellular proliferation in vitro and improved prostatic morphology in vivo, thereby decreasing tumor progression. Tempol reduced inflammation in preclinical models, and downregulated the initial inflammatory signaling through toll-like receptors, not always mediated by the MyD88 pathway. In addition, it upregulated i kappa B-alpha and i kappa B -beta levels, leading to a decrease in NF-kappa B, TNF-alpha, and other inflammatory markers. Tempol also influenced cell survival markers. ConclusionsTempol can be considered a beneficial therapy for PCa treatment owing to its anti-inflammatory and antiproliferative effects. Nevertheless, the action of tempol was different depending on the degree of the prostatic lesion in vivo and hormone reliance in vitro. This indicates that tempol plays a multifaceted role in the prostatic tissue environment. (AU)

• Processo FAPESP: 21/02108-0 - Terapia coadjuvante no câncer de próstata: análise celular e molecular da resposta inflamatória e angiogênica em linhagens celulares tumorais humanas e nos estágios inicial e tardio das lesões no modelo transgênico TRAMP
• Beneficiário: Valéria Helena Alves Cagnon Quitete
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/21647-6 - Microambiente do Adenocarcinoma de Próstata: efeito in vivo e in vitro do tempol sobre marcadores de inflamação, estresse oxidativo, proliferação e expressão de miRNAs
• Beneficiário: Isabela Maria Urra Rossetto
• Modalidade de apoio: Bolsas no Brasil - Doutorado