Metabolic alterations in Strongyloidiasis stool samples unveil potential biomarkers of infection

Autor(es)

Ribeiro Sotelo Montanhaur, Allan Daniel ; Lima, Estela de Oliveira ; Delafiori, Jeany ; Esteves, Cibele Zanardi ; Ribeiro Prado, Cesar Corat ; Allegretti, Silmara Marques ; Ueta, Marlene Tiduko ; Levy, Carlos Emilio ; Catharino, Rodrigo Ramos

• Tipo de documento: Artigo Científico
• Fonte: Acta Tropica; v. 227, p. 6-pg., 2022-03-01.

Resumo

Strongyloidiasis, a parasitosis caused by Strongyloides stercoralis in humans, is a very prevalent infection in tropical or subtropical areas. Gaps on public health strategies corroborates to the high global incidence of strongyloidiasis especially due to challenges involved on its diagnosis. Based on the lack of a gold-standard diagnostic tool, we aimed to present a metabolomic study for the assessment of stool metabolic alterations. Stool samples were collected from 25 patients segregated into positive for strongyloidiasis (n = 10) and negative control (n = 15) and prepared for direct injection high-resolution mass spectrometry analysis. Using metabolomics workflow, 18 metabolites were annotated increased or decreased in strongyloidiasis condition, from which a group of 5 biomarkers comprising caprylic acid, mannitol, glucose, lysophosphatidylinositol and hydroxy-dodecanoic acid demonstrated accuracy over 89% to be explored as potential markers. The observed metabolic alteration in stool samples indicates involvement of microbiota remodeling, parasite constitution, and host response during S. stercoralis infection. (AU)

• Processo FAPESP: 19/05718-3 - Determinação das alterações metabólicas e do potencial terapêutico do Zika Vírus em células tumorais por espectrometria de massas e inteligência artificial
• Beneficiário: Jeany Delafiori
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/07137-0 - Análise proteômica de Biomphalaria glabrata infectada por Schistosoma mansoni e Angiostrongylus cantonensis
• Beneficiário: Silmara Marques Allegretti
• Modalidade de apoio: Auxílio à Pesquisa - Regular